Kennedy D W, Aust S D, Bumpus J A
Biotechnology Center, Utah State University, Logan 84322-4430.
Appl Environ Microbiol. 1990 Aug;56(8):2347-53. doi: 10.1128/aem.56.8.2347-2353.1990.
The ability of Phanerochaete chrysosporium to degrade six alkyl halide insecticides (aldrin, dieldrin, heptachlor, chlordane, lindane, and mirex) in liquid and soil-corncob matrices was compared by using 14C-labeled compounds. Of these, only [14C]lindane and [14C]chlordane underwent extensive biodegradation, as evidenced by the fact that 9.4 to 23.4% of these compounds were degraded to 14CO2 in 30 days in liquid cultures and 60 days in soil-corncob cultures inoculated with P. chrysosporium. Although [14C]aldrin, [14C]dieldrin, [14C]heptachlor, and [14D]mirex were poorly mineralized, substantial bioconversion occurred, as determined by substrate disappearance and metabolite formation. Nonbiological disappearance was observed only with chlordane and heptachlor.
通过使用14C标记的化合物，比较了黄孢原毛平革菌在液体和土壤-玉米芯基质中降解六种卤代烷类杀虫剂（艾氏剂、狄氏剂、七氯、氯丹、林丹和灭蚁灵）的能力。其中，只有[14C]林丹和[14C]氯丹发生了广泛的生物降解，这一点可由以下事实证明：在接种了黄孢原毛平革菌的液体培养物中，30天内这些化合物有9.4%至23.4%降解为14CO2；在土壤-玉米芯培养物中，60天内有此降解情况。尽管[14C]艾氏剂、[14C]狄氏剂、[14C]七氯和[14C]灭蚁灵的矿化程度很低，但通过底物消失和代谢物形成确定发生了大量的生物转化。仅在氯丹和七氯中观察到非生物消失。
