Sayadi S, Zorgani F, Ellouz R
Centre de Biotechnologie de Sfax, Tunisia.
Appl Biochem Biotechnol. 1996 Mar;56(3):265-76. doi: 10.1007/BF02786957.
This paper describes the decolorization and chemical oxygen demand (COD) removal of olive mill waste-waters (OMW) by Phanerochaete chrysosporium grown in agitated submerged cultures. When P. chrysosporium was cultivated in the form of pellet, no decolorization of crude OMW was observed. Decolorization occurred only after removing by ultrafiltration, the high-mol-wt (HM) polyphenolic fraction (> 60 kDa). The use of high lignin peroxidase (LiP) producing medium yielded the highest levels of OMW decolorization and COD removal. In this case, extensive depolymerization and subsequent accumulation of phenolics with intermediates molecular weight were observed. Furthermore, increasing the concentration of the HM fraction decreased the color and COD removals. The decolorizing activity was lost when the concentration of the HM fraction reached 25% (v/v). Consequently, LiP activity was found to be completely inhibited in the presence of HM fraction, but not with the low-mol-wt (LM) polyphenolic fraction (< 8 kDa). The use of P. chrysosporium immobilized on polyurethane foam resulted in efficient decolorization of crude OMW. Moreover, the addition of an induction medium was shown to perform several repeated batch cultures for OMW decolorization and COD removal.
本文描述了在搅拌式深层培养中生长的黄孢原毛平革菌对橄榄果渣废水（OMW）的脱色及化学需氧量（COD）去除情况。当黄孢原毛平革菌以菌球形式培养时，未观察到粗制OMW的脱色现象。仅在通过超滤去除高分子量（HM）多酚级分（>60 kDa）后才发生脱色。使用产生高木质素过氧化物酶（LiP）的培养基可实现最高水平的OMW脱色和COD去除。在这种情况下，观察到了广泛的解聚以及随后中等分子量酚类物质的积累。此外，增加HM级分的浓度会降低颜色和COD去除率。当HM级分的浓度达到25%（v/v）时，脱色活性丧失。因此，发现LiP活性在存在HM级分的情况下会被完全抑制，但在存在低分子量（LM）多酚级分（<8 kDa）时不会。使用固定在聚氨酯泡沫上的黄孢原毛平革菌可实现粗制OMW的有效脱色。此外，添加诱导培养基可进行多次重复分批培养以实现OMW脱色和COD去除。
