Yamaue H, Tanimura H, Noguchi K, Hotta T, Tani M, Tsunoda T, Iwahashi M, Tamai M, Iwakura S
Department of Gastroenterological Surgery, Wakayama Medical College, Japan.
Br J Cancer. 1992 Nov;66(5):794-9. doi: 10.1038/bjc.1992.362.
A major problem associated with the chemosensitivity testing of fresh human tumour cells using the MTT assay is the contamination of nonmalignant cells in the tumour tissues. Highly purified fresh human gastric cancer cells could be obtained from 43 solid tumours and eight malignant ascites for the MTT assay. The success rate of the MTT assay was 87.9% (51 of the 58 cases), and the purity of tumour cells was greater than 90% after separation on Ficoll-Hypaque and Percoll discontinuous gradients in primary, or metastatic lesions, and also ascites. Cisplatin, mitomycin, and doxorubicin were more potent drugs than etoposide and 5-FU against gastric cancer cells. The chemosensitivity in differentiated cancer was equivalent to that in non-differentiated cancer. Twenty of the 51 patients with gastric cancer had evaluable lesions, and they received chemotherapy according to the results of the MTT assay using highly purified tumour cells. A clinical response was obtained in 12 of these 20 patients (response rate: 60.0%; five with complete response, seven with partial response).
使用MTT法对新鲜人肿瘤细胞进行化学敏感性测试所面临的一个主要问题是肿瘤组织中非恶性细胞的污染。可从43个实体瘤和8例恶性腹水样本中获取高度纯化的新鲜人胃癌细胞用于MTT法检测。MTT法检测的成功率为87.9%（58例中的51例），在原发病灶、转移病灶以及腹水中，经Ficoll-Hypaque和Percoll不连续梯度分离后，肿瘤细胞的纯度大于90%。顺铂、丝裂霉素和阿霉素对胃癌细胞的药效比依托泊苷和5-氟尿嘧啶更强。分化型癌的化学敏感性与未分化癌相当。51例胃癌患者中有20例具有可评估病灶，他们根据使用高度纯化肿瘤细胞的MTT检测结果接受了化疗。这20例患者中有12例获得了临床缓解（缓解率：60.0%；5例完全缓解，7例部分缓解）。
