Cho Nam Hoon, Choi Yoon Pyo, Moon Dong Suk, Kim Haeryoung, Kang Suki, Ding Owen, Rha Sun Young, Yang Yeon Ju, Cho Sang Ho
Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
Cancer Sci. 2006 Oct;97(10):1082-92. doi: 10.1111/j.1349-7006.2006.00292.x.
Cyclin A1 and cyclin B1 are overexpressed in various tumors but are present at low levels in normal tissues. Cyclin A1 is restricted to germ cells undergoing meiosis. In order to explore the possibility of using cyclin A1 and cyclin B1 as anticancer targets, we knocked them down in two lung cancer cell lines, H157 and H596, using siRNA. As with cyclin A1 siRNA in lung cancer cell lines, cyclin B1, Cdc2 and CDK2 were all significantly downregulated. The S phase fraction increased significantly, and they eventually underwent apoptosis by way of downregulated intrinsic apoptotic pathways and modulators with upregulated extrinsic apoptotic pathways. Our study suggests that cyclin A1 might be a promising anticancer target specific to lung cancer.
细胞周期蛋白A1（Cyclin A1）和细胞周期蛋白B1（Cyclin B1）在多种肿瘤中过表达，但在正常组织中含量较低。细胞周期蛋白A1局限于进行减数分裂的生殖细胞。为了探索将细胞周期蛋白A1和细胞周期蛋白B1用作抗癌靶点的可能性，我们使用小干扰RNA（siRNA）在两种肺癌细胞系H157和H596中敲低它们。与肺癌细胞系中的细胞周期蛋白A1 siRNA一样，细胞周期蛋白B1、细胞周期蛋白依赖性激酶2（Cdc2）和细胞周期蛋白依赖性激酶2（CDK2）均显著下调。S期比例显著增加，它们最终通过下调内在凋亡途径和调节因子以及上调外在凋亡途径而发生凋亡。我们的研究表明，细胞周期蛋白A1可能是一种有前景的肺癌特异性抗癌靶点。
