Yu P, Ledgerwood A M, Lucas C E
Department of Surgery, Wayne State University, Detroit, MI, USA.
Wound Repair Regen. 1997 Jan-Mar;5(1):62-8. doi: 10.1046/j.1524-475X.1997.50113.x.
Tendon gap closure was examined in a tissue culture model and found to have a similar time course as skin wound closure. Foot tendons from White Rock chickens were mounted in a collagen gel matrix and maintained with the use of Dulbecco's modified Eagle medium, containing fetal calf serum and antibiotics, for 4 weeks at 37 degrees C in an incubator. Gap distances between tendons were measured every 1 to 3 days and plotted against time as the contraction curve. After an initial lag period of 4 to 8 days, gap distance showed a progressive decrease. Gap closure rate was defined as the slope of the contraction curve, and it was found to be a function of initial gap distance (r = 0.643, p 0.045). The time necessary to reduce the initial gap distance by half had a significant correlation with the initial gap distance (r = 0.986, p < 0.001). Fibroblast migration began on days 2 to 3 after a 1- to 2-day lag period. Fibroblasts were visible in the tendon gap region before the start of collagen gel contraction. At this time, the fibroblast migration rate was 0.33 mm/day. A critical density of fibroblasts was necessary to start collagen gel contraction. Once the gap distance began to diminish, fibroblast migration measurements were hampered because the measurable area was decreasing. Collagen gel contraction reduced the measurable fibroblast migration rate by nearly half to 0.18 mm/day. A linear correlation was found between fibroblast distance traveled and time in culture during both the gel lag and gel contraction time periods. This tendon culture model may be potentially useful for wound healing studies because it allows for studies of fibroblast activity in the early lag phase when the cells populate the collagen lattice but before contraction of the gel occurs.
在组织培养模型中研究了肌腱间隙闭合情况，发现其时间进程与皮肤伤口闭合相似。将来自白洛克鸡的足部肌腱置于胶原凝胶基质中，并使用含有胎牛血清和抗生素的杜氏改良伊格尔培养基，于37℃在培养箱中维持4周。每隔1至3天测量肌腱之间的间隙距离，并将其作为收缩曲线随时间绘制。在最初4至8天的延迟期后，间隙距离逐渐减小。间隙闭合率定义为收缩曲线的斜率，发现它是初始间隙距离的函数（r = 0.643，p 0.045）。将初始间隙距离减半所需的时间与初始间隙距离具有显著相关性（r = 0.986，p < 0.001）。成纤维细胞迁移在1至2天的延迟期后的第2至3天开始。在胶原凝胶收缩开始之前，在肌腱间隙区域可见成纤维细胞。此时，成纤维细胞迁移率为0.33毫米/天。成纤维细胞的临界密度是启动胶原凝胶收缩所必需的。一旦间隙距离开始减小，由于可测量区域在减小，成纤维细胞迁移测量就受到阻碍。胶原凝胶收缩使可测量的成纤维细胞迁移率降低了近一半，降至0.18毫米/天。在凝胶延迟期和凝胶收缩期，成纤维细胞迁移的距离与培养时间之间发现了线性相关性。这种肌腱培养模型可能对伤口愈合研究具有潜在用途，因为它允许在细胞填充胶原晶格但凝胶收缩发生之前的早期延迟阶段研究成纤维细胞的活性。
