Krogerus L, Andersson L C
Department of Pathology, University of Helsinki, Finland.
Cancer. 1990 Oct 15;66(8):1802-9. doi: 10.1002/1097-0142(19901015)66:8<1802::aid-cncr2820660827>3.0.co;2-z.
The expression of glycoconjugates in primary tumors and their metastases in 18 consecutive cases of metastasized breast cancer was studied by use of lectin histochemistry. Paraffin sections were stained with a panel of seven fluorochrome-labeled lectins with defined sugar specificities. The study revealed variation in the lectin binding patterns of individual cancers. In the primary tumors the lectin reactivity was diversified, whereas in their respective metastases it was rather homogeneous. This finding indicates that there is intratumoral heterogeneity in the primary cancers, whereas the selected subclones of malignant cells with restricted glycoconjugate expression seem to give rise to metastases.
运用凝集素组织化学方法，对18例转移性乳腺癌原发肿瘤及其转移灶中糖缀合物的表达进行了研究。石蜡切片用一组七种具有特定糖特异性的荧光染料标记凝集素进行染色。研究揭示了各例癌症凝集素结合模式的差异。在原发肿瘤中，凝集素反应具有多样性，而在其各自的转移灶中则较为一致。这一发现表明，原发性癌症存在肿瘤内异质性，而糖缀合物表达受限的恶性细胞所选亚克隆似乎会引发转移。
