Louis C J, Sztynda T, Cheng Z M, Wyllie R G
Cancer. 1983 Oct 1;52(7):1244-50. doi: 10.1002/1097-0142(19831001)52:7<1244::aid-cncr2820520719>3.0.co;2-u.
Paraffin-embedded sections from a variety of breast lesions were stained with a number of lectins labeled with fluorescein isothiocyanate and examined by fluorescence microscopy: 95 non-neoplastic tissues and 69 malignant tumors were examined. Lectin binding was demonstrated in all malignant tumors, the fluorescence being confined to the plasma membrane of the tumor cells. Normal and hyperplastic tissues either failed to stain or showed a grossly diminished level of fluorescence. The significance of the staining reaction is discussed.
用异硫氰酸荧光素标记的多种凝集素对来自各种乳腺病变的石蜡包埋切片进行染色，并通过荧光显微镜检查：共检查了95份非肿瘤组织和69份恶性肿瘤。在所有恶性肿瘤中均显示出凝集素结合，荧光局限于肿瘤细胞的质膜。正常组织和增生组织要么未染色，要么荧光水平明显降低。文中讨论了染色反应的意义。
