Al-Adnani M S, Kirrane J A, McGee J O
Br J Cancer. 1975 Jun;31(6):653-60. doi: 10.1038/bjc.1975.112.
Thirty-two scirrhous cancers of breast have been examined to determine the origin of the collagen stroma in these tumours. Employing two immunohistochemical techniques it has been shown that the malignant epithelial cells in 30 of these tumours contain not only collagen but also prolyl hydroxylase, a key enzyme in collagen biosynthesis. Neither this enzyme nor collagen was detectable in the spindle cells in the stroma of these tumours. Neither the epithelium in normal breast, that in fibrocystic disease and in fibroadenomata, nor the malignant epithelium in two medullary cancers of breast contained either collagen or prolyl hydroxylase. These results strongly suggest that the malignant epithelium of scirrhous breast cancers produces its own collagen stroma and that the scirrhous reaction in these tumours is not a host response to tumour invasion. The production of collagen and prolyl hydroxylase by breast cancer cells (of the scirrhous type) therefore represents another example of inappropriate protein production by a human tumour.
对32例乳腺硬癌进行了检查，以确定这些肿瘤中胶原基质的来源。采用两种免疫组织化学技术表明，这些肿瘤中有30例的恶性上皮细胞不仅含有胶原蛋白，还含有脯氨酰羟化酶，这是胶原蛋白生物合成中的一种关键酶。在这些肿瘤基质中的梭形细胞中均未检测到这种酶和胶原蛋白。正常乳腺上皮、纤维囊性疾病和纤维腺瘤中的上皮，以及两例乳腺髓样癌中的恶性上皮均未含有胶原蛋白或脯氨酰羟化酶。这些结果有力地表明，乳腺硬癌的恶性上皮产生其自身的胶原基质，并且这些肿瘤中的硬癌反应不是宿主对肿瘤侵袭的反应。因此，乳腺癌细胞（硬癌类型）产生胶原蛋白和脯氨酰羟化酶是人类肿瘤不适当蛋白质产生的另一个例子。
