淋巴细胞中胶原蛋白生物合成活性的表达。

Expression of collagen biosynthetic activities in lymphocytic cells.

作者信息

Chen-Kiang S, Cardinale G J, Udenfriend S

出版信息

Proc Natl Acad Sci U S A. 1978 Mar;75(3):1379-83. doi: 10.1073/pnas.75.3.1379.

DOI:10.1073/pnas.75.3.1379

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411475/

Abstract

Immunoglobulin-producing Merwin plasma cells, MPC-11, have been found to contain proplyl hydroxylase (prolyl-glycyl-peptide,2-oxoglutarate:oxygen oxidoreductase; EC 1.14.11.2) activity and its crossreacting protein, as well as hydroxyproline and a collagenous protein that could not be classified as type I, II, or III collagen. Friend leukemic cells, on the other hand, contained only prolyl hydroxylase. Thymus-derived (T) lymphocytes and bone-marrow-derived (B) lymphocytes freshly isolated from BALB/c mice expressed low but significant prolyl hydroxylase activity. Upon stimulation with phytohemagglutinin, the enzyme activity in T cells increased 22- to 29-fold. Crossreacting protein was also increased and appeared more stable than the prolyl hydroxylase. The effect of lipopolysaccharide stimulation on B cells uas similar but not as pronounced. Thus, even when not accompanied by other collagen biosynthetic activities, prolyl hydroxylase is present in all cells of hematologic origin.

摘要

已发现产生免疫球蛋白的默温氏浆细胞（MPC-11）含有脯氨酰羟化酶（脯氨酰-甘氨酰-肽，2-氧代戊二酸：氧氧化还原酶；EC 1.14.11.2）活性及其交叉反应蛋白，以及羟脯氨酸和一种无法归类为I型、II型或III型胶原的胶原质蛋白。另一方面，弗瑞德白血病细胞仅含有脯氨酰羟化酶。从BALB/c小鼠新鲜分离的胸腺来源（T）淋巴细胞和骨髓来源（B）淋巴细胞表达低水平但显著的脯氨酰羟化酶活性。在用植物血凝素刺激后，T细胞中的酶活性增加了22至29倍。交叉反应蛋白也增加了，并且似乎比脯氨酰羟化酶更稳定。脂多糖刺激对B细胞的作用类似但不那么明显。因此，即使没有其他胶原生物合成活性相伴，脯氨酰羟化酶也存在于所有血液来源的细胞中。

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引用本文的文献

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本文引用的文献

1

USE OF COLLAGENASE TO IDENTIFY ENZYMATICALLY FORMED COLLAGEN AND A HYDROXYPROLINE-DEFICIENT INTERMEDIATE.

J Biol Chem. 1965 Jul;240:3099-103.

2

TRANSFORMATION OF PROPERTIES OF AN ESTABLISHED CELL LINE BY SV40 AND POLYOMA VIRUS.

Proc Natl Acad Sci U S A. 1964 Jan;51(1):66-73. doi: 10.1073/pnas.51.1.66.

3

COLLAGEN FORMATION IN VITRO BY ESTABLISHED MAMMALIAN CELL LINES.

Exp Cell Res. 1963 Aug;31:444-7. doi: 10.1016/0014-4827(63)90025-9.

4

Differentiated cell types and the regulation of collagen synthesis.

Nature. 1966 Nov 5;212(5062):631-3. doi: 10.1038/212631b0.

5

Collagen synthesis in fibroblasts transformed by oncogenic viruses.

Nature. 1966 Feb 26;209(5026):916-7. doi: 10.1038/209916a0.

6

Purification and properties of collagen proline hydroxylase from newborn rat skin.

Arch Biochem Biophys. 1970 Aug;139(2):329-39. doi: 10.1016/0003-9861(70)90485-6.

7

Biochemical studies on the structure of chick bone collagen.

Fed Proc. 1969 Nov-Dec;28(6):1839-45.

8

Synthesis of collagen by mammalian cell lines of fibroblastic and nonfibroblastic origin.

Proc Natl Acad Sci U S A. 1965 Jun;53(6):1360-5. doi: 10.1073/pnas.53.6.1360.

9

Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.

Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82. doi: 10.1073/pnas.68.2.378.

10

Collagen polymorphism: characterization of molecules with the chain composition (alpha 1 (3)03 in human tissues.

Science. 1974 Mar;183(130):1200-1. doi: 10.1126/science.183.4130.1200.

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