Chen-Kiang S, Cardinale G J, Udenfriend S
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1379-83. doi: 10.1073/pnas.75.3.1379.
Immunoglobulin-producing Merwin plasma cells, MPC-11, have been found to contain proplyl hydroxylase (prolyl-glycyl-peptide,2-oxoglutarate:oxygen oxidoreductase; EC 1.14.11.2) activity and its crossreacting protein, as well as hydroxyproline and a collagenous protein that could not be classified as type I, II, or III collagen. Friend leukemic cells, on the other hand, contained only prolyl hydroxylase. Thymus-derived (T) lymphocytes and bone-marrow-derived (B) lymphocytes freshly isolated from BALB/c mice expressed low but significant prolyl hydroxylase activity. Upon stimulation with phytohemagglutinin, the enzyme activity in T cells increased 22- to 29-fold. Crossreacting protein was also increased and appeared more stable than the prolyl hydroxylase. The effect of lipopolysaccharide stimulation on B cells uas similar but not as pronounced. Thus, even when not accompanied by other collagen biosynthetic activities, prolyl hydroxylase is present in all cells of hematologic origin.
已发现产生免疫球蛋白的默温氏浆细胞（MPC-11）含有脯氨酰羟化酶（脯氨酰-甘氨酰-肽，2-氧代戊二酸：氧氧化还原酶；EC 1.14.11.2）活性及其交叉反应蛋白，以及羟脯氨酸和一种无法归类为I型、II型或III型胶原的胶原质蛋白。另一方面，弗瑞德白血病细胞仅含有脯氨酰羟化酶。从BALB/c小鼠新鲜分离的胸腺来源（T）淋巴细胞和骨髓来源（B）淋巴细胞表达低水平但显著的脯氨酰羟化酶活性。在用植物血凝素刺激后，T细胞中的酶活性增加了22至29倍。交叉反应蛋白也增加了，并且似乎比脯氨酰羟化酶更稳定。脂多糖刺激对B细胞的作用类似但不那么明显。因此，即使没有其他胶原生物合成活性相伴，脯氨酰羟化酶也存在于所有血液来源的细胞中。
