Gao Xiu-xia, Du Hai-ke, Wang Yi-fei, Li Hong-wei
Affiliated Hospital of Medical College of Chinese People's Armed Polices Forces, Tianjin 300162,China.
Wei Sheng Yan Jiu. 2006 Jul;35(4):406-8.
To study the effect of Ligustrazine on type I, III collagen synthesis in lung tissue of silica-treated rat.
The 128 experimental rats were randomly divided into control, silica and Ligustrazine group. 1ml silica (50g/L) was injected intratracheally in silica group and Ligustrazine group, while Ligustrazine group were injected intraperitoneally 50mg/(kg x d) Ligustrazine. Samples were collected on the 1st, 3rd, 7th, 14th, 21st, 28th day after injected silica. Type I , III collagen on paraffin-embedded lung sections were stained with sirius red, detected by polarized light microscopy and quantified by Image-Pro Plus.
On the 3rd day after silica instillation, type III collagen began to appear, while type I collagen formed on the 7th day. Thereafter Type I, III collagen increased progressively. Compared with control group, type I, III collagen expression of silica group increased. At different time points type I, III collagen expression of Ligustrazine group decreased than silica group. There was significant difference of type I collagen area percentage on the 7th, 14th, 28th day, type III collagen area percentage on the 28th day between Ligustrazine group and silica group.
Silica dioxide could induce the increase of type I, III collagen production in lung tissue, and their stages are different. Ligustrazine could suppress type I, III collagen production in lung tissue of silica-treated rat, and it's mechanism would be studied.
研究川芎嗪对二氧化硅处理大鼠肺组织Ⅰ型、Ⅲ型胶原蛋白合成的影响。
将128只实验大鼠随机分为对照组、二氧化硅组和川芎嗪组。二氧化硅组和川芎嗪组经气管内注入1ml二氧化硅（50g/L），川芎嗪组同时腹腔注射50mg/(kg·d)川芎嗪。在注入二氧化硅后的第1、3、7、14、21、28天采集样本。用天狼星红对石蜡包埋的肺组织切片上的Ⅰ型、Ⅲ型胶原蛋白进行染色，通过偏光显微镜检测并用Image-Pro Plus软件进行定量分析。
二氧化硅注入后第3天开始出现Ⅲ型胶原蛋白，第7天形成Ⅰ型胶原蛋白。此后Ⅰ型、Ⅲ型胶原蛋白逐渐增加。与对照组相比，二氧化硅组Ⅰ型、Ⅲ型胶原蛋白表达增加。在不同时间点，川芎嗪组Ⅰ型、Ⅲ型胶原蛋白表达低于二氧化硅组。川芎嗪组与二氧化硅组在第7、14、28天的Ⅰ型胶原蛋白面积百分比以及第28天的Ⅲ型胶原蛋白面积百分比存在显著差异。
二氧化硅可诱导肺组织中Ⅰ型、Ⅲ型胶原蛋白生成增加，且其阶段不同。川芎嗪可抑制二氧化硅处理大鼠肺组织中Ⅰ型、Ⅲ型胶原蛋白的生成，其机制有待进一步研究。
