Xu Zheng-rong, Du Hai-ke, Wang Shi-xin, Liu Dian-wu, Chang Ai-hua
Department of Preventive Medicine of Hebei North University, ZhangJiaKou, Hebei Province 075000, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2006 Sep;24(9):544-6.
To evaluate the effects of taurine in diet on the expression of type I and III collagen and collagen ratio at different time points in rats lung by image process technology.
Wistar rats were randomly divided into three groups: the saline instilled with a control diet (the saline treated group); silica instilled with a control diet (the silica treated group); and silica instilled with a diet containing 2.5% taurine (the taurine treated group). Animal models were established by the direct tracheal instillation of silica into rat lungs exposed surgically. The taurine concentration of serum was analyzed by means of HPLC. Paraffin embedded lung sections were stained with Sirius red. Polarization microscopy and Image Pro Plus Version 4.5 for windows were used for detecting type I and III collagen.
The concentration of taurine in serum of the taurine treated group was significantly elevated compared to the saline treated and silica treated group (P < 0.05 or P < 0.01). Sirius red polarization microscopy showed that type I and III collagen positive area percentage were elevated in the silica treated rats compared with the saline treated group. On the 7th, 14th, 21st, 28th day after silica instillation type I collagen positive area percentage was increased by 3.84, 3.77, 3.73, 9.83 respectively (P < 0.01), and type III collagen positive area percentage were elevated by a little in the silica treated rats compared with saline treated group. The taurine treatment significantly decreased elevation of silica type I collagen positive area percentage of lung by 2.39, 1.62, 7.13 at the 7th, 21st, 28th day respectively (P < 0.05 or P < 0.01), and type III collagen positive area percentage of lung by 2.62 at the 28th day (P < 0.05) compared with the silica treated group. The ratio of type I to III collagen was increased from the 7th day to 28th day after silica instillation, and reached 1.87 at the 28th day with the maximal ratio in the silica-treated group.
Treatment with taurine can effectively attenuate type I and III collagen expression in the rat lung induced by silica particles at different time points in our study.
采用图像分析技术评价饮食中牛磺酸对大鼠肺组织不同时间点Ⅰ型和Ⅲ型胶原蛋白表达及胶原蛋白比例的影响。
将Wistar大鼠随机分为三组：气管内注入生理盐水并给予对照饮食（生理盐水处理组）；气管内注入二氧化硅并给予对照饮食（二氧化硅处理组）；气管内注入二氧化硅并给予含2.5%牛磺酸的饮食（牛磺酸处理组）。通过手术暴露大鼠肺部后直接经气管注入二氧化硅建立动物模型。采用高效液相色谱法分析血清中牛磺酸浓度。肺组织石蜡切片用天狼星红染色。利用偏光显微镜和Windows版Image Pro Plus 4.5软件检测Ⅰ型和Ⅲ型胶原蛋白。
与生理盐水处理组和二氧化硅处理组相比，牛磺酸处理组血清中牛磺酸浓度显著升高（P<0.05或P<0.01）。天狼星红偏光显微镜观察显示，与生理盐水处理组相比，二氧化硅处理组大鼠肺组织中Ⅰ型和Ⅲ型胶原蛋白阳性面积百分比升高。在注入二氧化硅后的第7、14、21、28天，Ⅰ型胶原蛋白阳性面积百分比分别增加3.84、3.77、3.73、9.83（P<0.01），与生理盐水处理组相比，二氧化硅处理组大鼠肺组织中Ⅲ型胶原蛋白阳性面积百分比略有升高。与二氧化硅处理组相比，牛磺酸处理在第7、21、28天分别使二氧化硅诱导的肺组织Ⅰ型胶原蛋白阳性面积百分比显著降低2.39、1.62、7.13（P<0.05或P<0.01），在第28天使Ⅲ型胶原蛋白阳性面积百分比降低2.62（P<0.05）。从注入二氧化硅后的第7天到第28天，Ⅰ型与Ⅲ型胶原蛋白的比例增加，在第28天达到1.87，为二氧化硅处理组中的最大比例。
在本研究中，牛磺酸处理可有效减轻二氧化硅颗粒在不同时间点诱导的大鼠肺组织中Ⅰ型和Ⅲ型胶原蛋白的表达。
