Xu Zhengrong, Du Haike, Wang Shixin, Liu Dianwu
Department of Preventive Medicine of Hebei North University, Zhangjiakou 075000, China.
Wei Sheng Yan Jiu. 2007 May;36(3):364-6.
To evaluate the effects of taurine on the expression of tumor necrosis factor-alpha (TNF-alpha) protein of lung in rats treated by silica.
144 Wistar rats were randomly divided into three experimental groups: saline-instilled with a control diet (saline-treated group), silica-instilled with a control diet (silica-treated group), and silica-instilled with a diet containing 2.5% taurine (taurine-treated group). There were 48 rats in every experimental group. Rats were treated by direct tracheal instillation of silica into rat lungs exposed surgically. 8 rats in every experimental group were killed at each at 6 time points (1, 3, 7, 14, 21 and 28 day) respectively. The taurine concentration of serum were analyzed by means of HPLC. The expression of TNF-alpha protein in paraffin-embedded lung sections with Streptavidin/ peroxidase(SP) immunohistochemistry on tissue microarray were measured by image-pro Plus.
The concentration of taurine in serum of taurine-treated group were significantly elevated when compared with saline-treated and silica-treated groups (P < 0.05, P < 0.01). After instillation of silica, TNF-alpha positive area percent of the lung was elevated in silica-treated rats when compared with the corresponding saline-treated group, peaking at 7 days, and elevated by 6.57% and 2.37% at 7 days and 14 days respectively (P < 0.01 or P < 0.05). Taurine treatment significantly decreased silica-elevated TNF-alpha positive area percent by 3.52 at 7 day (P < 0.05).
Treatment with taurine can effectively attenuate the pathological expression of TNF-alpha protein of lung in rats induced by silica.
评估牛磺酸对二氧化硅处理的大鼠肺组织中肿瘤坏死因子-α（TNF-α）蛋白表达的影响。
144只Wistar大鼠随机分为三个实验组：生理盐水灌注并给予对照饮食（生理盐水处理组）、二氧化硅灌注并给予对照饮食（二氧化硅处理组）、二氧化硅灌注并给予含2.5%牛磺酸的饮食（牛磺酸处理组）。每个实验组有48只大鼠。通过手术暴露大鼠肺部后直接经气管向大鼠肺内灌注二氧化硅进行处理。每个实验组分别在6个时间点（1、3、7、14、21和28天）各处死8只大鼠。采用高效液相色谱法分析血清中牛磺酸浓度。通过图像分析软件在组织芯片上用链霉亲和素/过氧化物酶（SP）免疫组化法检测石蜡包埋肺组织切片中TNF-α蛋白的表达。
与生理盐水处理组和二氧化硅处理组相比，牛磺酸处理组血清中牛磺酸浓度显著升高（P<0.05，P<0.01）。灌注二氧化硅后，与相应的生理盐水处理组相比，二氧化硅处理的大鼠肺组织中TNF-α阳性面积百分比升高，在第7天达到峰值，在第7天和第14天分别升高6.57%和2.37%（P<0.01或P<0.05）。牛磺酸处理在第7天显著降低了二氧化硅升高的TNF-α阳性面积百分比，降低了3.52（P<0.05）。
牛磺酸处理可有效减轻二氧化硅诱导的大鼠肺组织中TNF-α蛋白的病理表达。
