Caignard A, Genne P, Olsson N O, Oriol R, Martin F
INSERM U.252, Faculty of Medicine, Dijon, France.
Int J Cancer. 1990 Oct 15;46(4):633-9. doi: 10.1002/ijc.2910460414.
Cell variants that differ in their tumorigenicity and immunogenicity have been isolated from a BDIX rat colon adenocarcinoma cell line, DHD/K12. One variant, PRO, and the clones derived from it, are poorly immunogenic and induce progressive and metastatic tumors; the other one, REG, and its clones, are highly immunogenic and induce regressive tumors. When looking for a membrane marker distinguishing between PRO and REG lines, we obtained monoclonal hybridomas by immunizing BALB/c mice with PROb or REGb cell clones. Hybridoma F11C, producing an IgM monoclonal antibody (MAb) was able to distinguish between the cell variants on membrane immunofluorescence. All REGb cells strongly express F11C membrane antigen. On PROb cells, F11C antigen expression is weak, as demonstrated by cytofluorimetric analysis, and limited to a fraction of the cell population. The F11C membrane antigen is highly specific for the DHD/K12 cell line and the variants derived from it, but is not expressed on cells dissociated from the DHD transplanted tumor, from which DHD/K12 was established, suggesting that F11C antigen emerged during cell culture. Fluorescence absorption on synthetic oligosaccharides demonstrated that F11C antibody cross-reacts with A type 3, A type 4 and A type 5 chain blood group tetrasaccharides.
已从BDIX大鼠结肠腺癌细胞系DHD/K12中分离出具有不同致瘤性和免疫原性的细胞变体。一种变体PRO及其衍生克隆免疫原性较差，可诱导进行性和转移性肿瘤；另一种变体REG及其克隆具有高度免疫原性，可诱导肿瘤消退。在寻找区分PRO和REG细胞系的膜标志物时，我们用PROb或REGb细胞克隆免疫BALB/c小鼠，获得了单克隆杂交瘤。产生IgM单克隆抗体（MAb）的杂交瘤F11C能够通过膜免疫荧光区分细胞变体。所有REGb细胞均强烈表达F11C膜抗原。通过细胞荧光分析表明，PROb细胞上F11C抗原表达较弱，且仅限于一部分细胞群体。F11C膜抗原对DHD/K12细胞系及其衍生变体具有高度特异性，但在从建立DHD/K12的DHD移植瘤中解离出的细胞上不表达，这表明F11C抗原是在细胞培养过程中出现的。对合成寡糖的荧光吸收表明，F11C抗体与A3型、A4型和A5型血型四糖发生交叉反应。
