Pelletier H, Olsson N O, Shimizu T, Lagadec P, Fady C, Reisser D, Jeannin J F
Research Group on Digestive Tumors, INSERM U.252, Faculty of Medicine, University of Dijon, France.
Immunobiology. 1987 Sep;175(3):202-13. doi: 10.1016/S0171-2985(87)80029-3.
In a previous work, a cell line (DHD/K12) was established from a colon adenocarcinoma induced in a BDIX rat by 1,2-dimethylhydrazine. From this line, two cloned sublines, PROb and REGb, were then isolated. When subcutaneously inoculated into syngeneic rats, PROb cells yield progressive tumors, whereas REGb cells yield tumors which regress. In this study, in a 16-h 51Cr release assay, natural cytotoxicity mediated by BDIX splenic nonadherent lymphoid cells (NK cells) was shown to be much higher against REGb cells than against PROb cells. Whatever the target cells, NK cytotoxicity was always higher when the effector cells were obtained from males rather than from females. Treatment of BDIX splenic lymphocytes by anti-asGM1 serum plus complement revealed that both anti-asGM1 sensitive and non-sensitive NK cells exist. The activity of anti-asGM1 non-sensitive NK cells appeared to be minor and to be detected only when the level of cytotoxicity before treatment was sufficiently high. The difference between PROb and REGb tumor growth appears to be linked, at least in part, to a higher sensitivity of REGb cells to NK cells and especially to anti-asGM1-sensitive NK cells.
在之前的一项研究中，从一只由1,2 - 二甲基肼诱导产生结肠腺癌的BDIX大鼠体内建立了一种细胞系（DHD/K12）。然后从该细胞系中分离出两个克隆亚系，即PROb和REGb。当皮下接种到同基因大鼠体内时，PROb细胞会形成进行性肿瘤，而REGb细胞形成的肿瘤会消退。在本研究中，在一项16小时的51Cr释放试验中，结果显示BDIX脾非黏附性淋巴细胞（NK细胞）介导的对REGb细胞的天然细胞毒性比对PROb细胞的要高得多。无论靶细胞是什么，当效应细胞取自雄性而非雌性时，NK细胞毒性总是更高。用抗asGM1血清加补体处理BDIX脾淋巴细胞后发现，既存在抗asGM1敏感的NK细胞，也存在不敏感的NK细胞。抗asGM1不敏感NK细胞的活性似乎较小，只有在处理前细胞毒性水平足够高时才能检测到。PROb和REGb肿瘤生长的差异似乎至少部分与REGb细胞对NK细胞尤其是对抗asGM1敏感的NK细胞的更高敏感性有关。
