Ha Jiyeon, Engler Cady R, Wild James R
Department of Biological and Agricultural Engineering, Texas A&M University, College Station, TX 77843-2117, USA.
Bioresour Technol. 2007 Jul;98(10):1916-23. doi: 10.1016/j.biortech.2006.07.038. Epub 2006 Sep 20.
Chlorferon and diethylthiophosphate (DETP) are the hydrolysis products of coumaphos, an organophosphate pesticide. In this research, two consortia of bacterial cultures, one responsible for degrading chlorferon and the other for degrading DETP, were selectively enriched from waste cattle dip solution. The enriched cultures were used as inocula to grow biomass for biodegradation studies. For chlorferon degradation, the optimum biomass concentration was found to be 80g/L, and pH 7.5 was selected as the optimal operating pH. Chlorferon degradation was characterized by substrate inhibition kinetics with parameter values estimated to be V(m)=0.062+/-0.011mg/(g-biomass)h, K(m)=21+/-7mg/L, and K(Si)=118+/-45mg/L. For DETP degradation, the optimum biomass concentration was found to be 60g/L, and the optimum pH was in the range of 7.5-8. DETP degradation was characterized by Michaelis-Menten kinetics with parameter values estimated to be V(m)=1.52+/-0.10mg/(g-biomass)h and K(m)=610+/-106mg/L.
氯甲磷和二乙基硫代磷酸酯（DETP）是有机磷农药蝇毒磷的水解产物。在本研究中，从废弃的牛药浴液中选择性富集了两个细菌培养物群落，一个负责降解氯甲磷，另一个负责降解DETP。将富集培养物用作接种物来培养生物量以进行生物降解研究。对于氯甲磷降解，发现最佳生物量浓度为80g/L，并选择pH 7.5作为最佳操作pH。氯甲磷降解的特征是底物抑制动力学，参数值估计为V(m)=0.062±0.011mg/(g-生物量)h、K(m)=21±7mg/L和K(Si)=118±45mg/L。对于DETP降解，发现最佳生物量浓度为60g/L，最佳pH范围为7.5 - 8。DETP降解的特征是米氏动力学，参数值估计为V(m)=1.52±0.10mg/(g-生物量)h和K(m)=610±106mg/L。
