Pelissier A, Ouhayoun J P, Sawaf M H, Forest N
Laboratoire de Biologie-Odontologie Université Paris VII, France.
J Biol Buccale. 1990 Jun;18(2):99-108.
Cytokeratin expression by the developing human enamel organ between the 10th and the 23rd gestational week was studied by indirect immunofluorescence microscopy technique using a panel of 15 monoclonal antibodies. The results showed that five antibodies (RKSE 60, Kk 8-60, EE 21-6, 6B10 and 1 C-7) were never reactive, that five antibodies (RCK 102, 42.39.13.1, Ks 19 and Pan 1-8) were always positive and that five antibodies (KB 37, RPN 11-62, Ks 13.1, Ks 8-12 and Ks 18.174) obtained or increased their positivity between weeks 12 and 13. It was concluded that a switch in cytokeratin expression occurred around the 12th-13th weeks. No further important change could be noticed after this period. So it is suggested that final cell differentiation was initiated at weeks 12-13.
利用一组15种单克隆抗体，采用间接免疫荧光显微镜技术，研究了妊娠第10至23周发育中的人牙釉质器官的细胞角蛋白表达。结果显示，5种抗体（RKSE 60、Kk 8 - 60、EE 21 - 6、6B10和1 C - 7）从未产生反应，5种抗体（RCK 102、42.39.13.1、Ks 19和Pan 1 - 8）始终呈阳性，5种抗体（KB 37、RPN 11 - 62、Ks 13.1、Ks 8 - 12和Ks 18.174）在第12至13周时获得阳性或阳性增强。得出结论，细胞角蛋白表达的转换发生在第12至13周左右。在此之后未观察到进一步的重要变化。因此提示最终的细胞分化在第12至13周开始。
