Smith A J, Wilson C, Matthews J B
Department of Oral Surgery and Oral Pathology, University of Birmingham Dental School, UK.
Histochemistry. 1990;94(3):329-35. doi: 10.1007/BF00266636.
The cytokeratin distribution in the developing rat enamel organ from day 15 of gestation through to 11 days post partum was examined immunohistochemically using a panel of monoclonal antibodies. A temporo-spatial programme of keratin expression was observed during odontogenesis and positive reactivity of the enamel organ was seen with the pan keratin antibodies CK1 (clone LP34 - reacts with a number of keratins including 6 and 18) and AE1-3 (reacts with most acidic and basic keratins). No reactivity was observed in the enamel organ with the other antibodies examined (Ks 8.12 [reacts with keratins 13 and 16], Ks 8.60 [reacts with keratins 10 and 11) and MCA157 [reacts with rat liver antigen]), although these antibodies did stain other epithelial tissues. This study supports the view that the epithelial cells of the enamel organ synthesize a tissue-specific subset of keratins which are related to the differentiation of the cells.
使用一组单克隆抗体，通过免疫组织化学方法检测了从妊娠第15天到产后11天发育中的大鼠牙釉质器官中的细胞角蛋白分布。在牙齿发生过程中观察到了角蛋白表达的时空程序，并且牙釉质器官与泛角蛋白抗体CK1（克隆LP34 - 与包括6和18在内的多种角蛋白反应）和AE1-3（与大多数酸性和碱性角蛋白反应）呈现阳性反应。在所检测的其他抗体（Ks 8.12 [与角蛋白13和16反应]、Ks 8.60 [与角蛋白10和11反应]以及MCA157 [与大鼠肝脏抗原反应]）中，牙釉质器官未观察到反应性，尽管这些抗体确实对其他上皮组织进行了染色。这项研究支持了牙釉质器官的上皮细胞合成与细胞分化相关的组织特异性角蛋白亚群的观点。
