Domingues M G, Jaeger M M, Araújo V C, Araújo N S
Department of Oral Pathology, School of Dentistry, Ibirapuera University, São Paulo, SP, Brazil.
Eur J Oral Sci. 2000 Feb;108(1):43-7. doi: 10.1034/j.1600-0722.2000.00717.x.
Cytokeratin (CK) is a filament which plays a central role in epithelial tissue and, like the polypeptides of intermediate filaments in general, shows a high degree of tissue specificity. The CK expression patterns of odontogenic epithelia are still poorly described. We studied the distribution of individual CK polypeptides in the human enamel organ at bell stage and in remnants of the dental lamina. Our immunohistochemical study showed that epithelial cells stained for CKs 7, 13, 14 and 19 with slight changes in their pattern during the differentiation phase of odontogenesis. There was negative staining for all other CK polypeptides tested (CKs 8, 10, 16, 17 and 18). Most of the CKs in the enamel organ epithelia did not show differences related to the stage-specific state of differentiation, except for CKs 14 and 19 at the inner enamel epithelium. A strong label for CK 14 was present at the inner dental epithelium at early bell stage, and this was substituted by CK 19 at the late bell stage when the ameloblasts were fully differentiated.
细胞角蛋白（CK）是一种在上皮组织中起核心作用的细丝，并且与一般中间丝的多肽一样，具有高度的组织特异性。牙源性上皮的CK表达模式仍描述甚少。我们研究了人钟状期釉器和牙板残余物中单个CK多肽的分布。我们的免疫组织化学研究表明，上皮细胞在牙胚发生分化阶段对CK7、13、14和19染色，其模式略有变化。对所有其他测试的CK多肽（CK8、10、16、17和18）呈阴性染色。釉器上皮中的大多数CK与分化的阶段特异性状态无关，除了内釉上皮中的CK14和19。在钟状早期，内牙上皮有强烈的CK14标记，当成釉细胞完全分化时，在钟状晚期被CK19取代。
