Perlman S, Evans G, Afifi A
Department of Pediatrics, University of Iowa, Iowa City 52242.
J Exp Med. 1990 Oct 1;172(4):1127-32. doi: 10.1084/jem.172.4.1127.
Previous results suggested that, after intranasal inoculation, mouse hepatitis virus (MHV), a neurotropic coronavirus, entered the central nervous system (CNS) via the olfactory and trigeminal nerves. To prove this hypothesis, the effect of interruption of the olfactory pathway on spread of the virus was studied using in situ hybridization. Unilateral surgical ablation of this pathway prevented spread of the virus via the olfactory tract on the side of the lesion. MHV RNA could be detected, however, at distal sites on the operated side, indicating that the virus spread via well-described circuits involving the anterior commissure from the control (intact) side of the brain. Viral transport via the trigeminal nerve was not affected by removal of the olfactory bulb, showing that the surgical procedure was specific for the olfactory pathway. These results prove conclusively that MHV gains entry to the CNS via a transneuronal route, and spreads to additional sites in the brain via known neuroanatomic pathways.
先前的研究结果表明，经鼻接种后，嗜神经冠状病毒小鼠肝炎病毒（MHV）通过嗅觉神经和三叉神经进入中枢神经系统（CNS）。为了验证这一假设，利用原位杂交技术研究了嗅觉通路中断对病毒传播的影响。对该通路进行单侧手术切除可阻止病毒通过病变侧的嗅束传播。然而，在手术侧的远端部位可检测到MHV RNA，这表明病毒通过大脑对照（完整）侧涉及前连合的既定回路进行传播。切除嗅球并不影响病毒通过三叉神经的运输，这表明该手术操作对嗅觉通路具有特异性。这些结果确凿地证明，MHV通过跨神经元途径进入中枢神经系统，并通过已知的神经解剖学通路传播至大脑的其他部位。
