Ouhayoun J P, Goffaux J C, Sawaf M H, Shabana A H, Collin C, Forest N
Laboratoire de Biologie-Odontologie, Université Paris, France.
J Periodontal Res. 1990 Sep;25(5):283-92. doi: 10.1111/j.1600-0765.1990.tb00917.x.
Cytokeratins represent specific markers of certain pathways of epithelial differentiation. The purpose of this study was to describe the alterations of cytokeratin pattern and topographical distribution of individual cytokeratins in inflamed gingiva. Five healthy and 15 inflammatory samples of human gingiva were studied. From each biopsy, cryostat sections allowed histological staining, immunofluorescence microscopy using a battery of monoclonal antibodies to cytokeratins, and gel electrophoresis. The results show marked differences in cytokeratin expression by healthy epithelia as compared with inflamed gingiva: in suprabasal cell layers there were reductions or disappearance of cytokeratins 1, 2 and 10, 11--specific for terminal differentiation--and increased expression of cytokeratins 4 and 13, as well as--in basal and parabasal cell layers--expression of cytokeratin 19. These alterations might represent an adaptation of involved epithelia to the alterations brought about by the inflammatory process.
细胞角蛋白是上皮分化特定途径的特异性标志物。本研究的目的是描述炎症牙龈中细胞角蛋白模式的改变以及各个细胞角蛋白的拓扑分布。研究了5例健康人牙龈样本和15例炎症牙龈样本。从每例活检组织中，用低温恒温器切片进行组织学染色、使用一系列抗细胞角蛋白单克隆抗体的免疫荧光显微镜检查以及凝胶电泳。结果显示，与炎症牙龈相比，健康上皮细胞中细胞角蛋白的表达存在显著差异：在基底上层细胞层中，细胞角蛋白1、2以及对终末分化具有特异性的细胞角蛋白10、11减少或消失，细胞角蛋白4和13的表达增加，并且在基底和副基底细胞层中，细胞角蛋白19表达。这些改变可能代表受累上皮细胞对炎症过程所带来改变的一种适应。
