Feghali-Assaly M, Sawaf M H, Serres G, Forest N, Ouhayoun J P
Laboratoire de Biologie-Odontologie, Institut Biomédical des Cordeliers, Toulouse, France.
J Periodontal Res. 1994 May;29(3):185-95. doi: 10.1111/j.1600-0765.1994.tb01212.x.
This study uses cytokeratins (CK) as markers to investigate the phenotype of the junctional epithelium (JE) in partially erupted human teeth. The gingival samples, which were clinically healthy, were carefully dissected from the teeth. Cryostat sections were cut for histological staining, immunofluorescence microscopy and gel electrophoresis. Cytokeratins were extracted after microdissection. The basal and suprabasal epithelial cell markers, cytokeratins 4, 5, 13, 14 and 19 were detected with specific monoclonal antibodies. They showed that the junctional epithelium in erupting teeth has a complex topography. The cytokeratin immunohistochemical profile distinguished between the primary junctional epithelium (CK 5, 14 and 19 in basal and suprabasal cells and CK 13 faintly stained throughout the suprabasal layers) and the adjacent epithelium that had the same cytokeratin profile as the sulcular epithelium (CK 5, 14 and 19 in basal cells and CK 4 and 13 intensively stained in the suprabasal cells). Extraction, two-dimensional electrophoresis and western blotting showed that this transitional JE during eruption also contained CK 6, 16 and perhaps CK 4. Thus, the JE in erupting teeth shows patterns of CK distribution that are very similar to that of developing oral epithelia.
本研究使用细胞角蛋白（CK）作为标志物，以研究部分萌出的人牙齿中结合上皮（JE）的表型。从临床上健康的牙齿小心地切取牙龈样本。制作低温恒温器切片用于组织学染色、免疫荧光显微镜检查和凝胶电泳。在显微切割后提取细胞角蛋白。用特异性单克隆抗体检测基底和基底上层上皮细胞标志物细胞角蛋白4、5、13、14和19。结果显示，正在萌出牙齿中的结合上皮具有复杂的形态。细胞角蛋白免疫组织化学图谱区分了原发性结合上皮（基底和基底上层细胞中的CK 5、14和19，基底上层各层中CK 13呈微弱染色）和与龈沟上皮具有相同细胞角蛋白图谱的相邻上皮（基底细胞中的CK 5、14，基底上层细胞中CK 4和13呈强染色）。提取、二维电泳和蛋白质印迹显示，萌出过程中的这种过渡性结合上皮还含有CK 6、16，可能还有CK 4。因此，正在萌出牙齿中的结合上皮显示出与发育中的口腔上皮非常相似的CK分布模式。
