Liu Jing, Yu Jinshu, Wang Ming, Liu Qun, Zhang Wei, Deng Chong, Ding Jun
Parasitology Laboratory, Department of Veterinary Preventive Medicine, College of Veterinary Medicine, China Agricultural University, Beijing, PR China.
Vet Parasitol. 2007 Feb 28;143(3-4):358-63. doi: 10.1016/j.vetpar.2006.08.034. Epub 2006 Sep 20.
The truncated NcSRS2 gene (tNcSRS2) by removal of the N-terminal hydrophobic sequence was cloned into the pGEX-6p-1 plasmid and subsequently expressed as a glutathione-S-transferase (GST) fusion protein. The purified recombinant tNcSRS2 protein was specific to Neospora caninum and was used in an ELISA for the diagnosis of neosporosis. There was a good agreement between tNctSRS2-based ELISA and three commercially available diagnostic kits (IDEXX ELISA, HIPRA ELISA and VMRD IFAT). Three hundred dairy cattle serum samples from 9 regions were tested by our ELISA. The herd prevalence was 100% and the overall prevalence was 20.3%. There was a statistically significant difference in seroprevalence between regions (P<0.01) and an association between abortion history and N. caninum seropositivity. Our study showed that neosporosis in dairy cattle is widespread in China.
通过去除N端疏水序列截短的NcSRS2基因（tNcSRS2）被克隆到pGEX-6p-1质粒中，随后作为谷胱甘肽-S-转移酶（GST）融合蛋白表达。纯化的重组tNcSRS2蛋白对犬新孢子虫具有特异性，并用于酶联免疫吸附测定（ELISA）以诊断新孢子虫病。基于tNctSRS2的ELISA与三种市售诊断试剂盒（IDEXX ELISA、HIPRA ELISA和VMRD间接荧光抗体试验）之间具有良好的一致性。用我们的ELISA检测了来自9个地区的300份奶牛血清样本。牛群流行率为100%，总体流行率为20.3%。各地区之间的血清阳性率存在统计学显著差异（P<0.01），并且流产史与犬新孢子虫血清阳性之间存在关联。我们的研究表明，奶牛新孢子虫病在中国广泛存在。
