Holt C, Parker T G, Dalgleish D G
Biochim Biophys Acta. 1975 Aug 19;400(2):283-92. doi: 10.1016/0005-2795(75)90183-x.
A method of measuring an average particle radius in a highly polydisperse dispersion using the wavelength dependence of turbidity is described. For particles which are no larger than 0.3 of the wavelength of light used, a polynomial representation of the scattering cross-section can be used. For larger particles, more extensive numerical calculations are required. The use of the method is illustrated by determining the average particle radius of casein micelles by both elastic and quasi-elastic light scattering techniques. A polydisperse homogeneous sphere model is found to be a reasonably accurate representation of casein micelles. Several modifications of the model which would improve the agreement between the two techniques are mentioned briefly.
描述了一种利用浊度的波长依赖性来测量高度多分散分散体系中平均颗粒半径的方法。对于不大于所用光波长0.3倍的颗粒，可以使用散射截面的多项式表示。对于较大的颗粒，则需要更广泛的数值计算。通过弹性和准弹性光散射技术测定酪蛋白胶束的平均颗粒半径来说明该方法的应用。发现多分散均匀球体模型是酪蛋白胶束的一种相当准确的表示。简要提及了该模型的几种改进，这些改进将提高两种技术之间的一致性。
