Fujita T, Sugimoto N, Yokoi F, Ohtsubo Y, Ikutoh M, Kato Y, Natsuhara Y, Oka S, Yano I
Osaka Research Institute, Sawai Pharmaceutical Co., Ltd.
Microbiol Immunol. 1990;34(6):523-32. doi: 10.1111/j.1348-0421.1990.tb03169.x.
The immunomodifying activity of a novel mycoloyl glycolipid, trehalose 2,3,6'-trimycolate (GaGM), from a unique psychrophilic acid-fast bacterium, Rhodococcus aurantiacus, was examined. ICR mice were primed intravenously (i.v.) or intraperitoneally (i.p.) with liposomes containing GaGM (300 micrograms/mouse), and were administered LPS dissolved in saline (25 micrograms/mouse, i.v.) 2 weeks later. Two hours after injection of LPS, interferons (IFNs) and tumor necrosis factor (TNF) were induced significantly in mice sera. The increase in activities of IFNs and TNF was approximately paralleled with granuloma formation in spleen of mice primed with GaGM. However, IFNs and TNF were not induced either in mice primed with GaGM but not elicited with LPS, or in those primed with GaGM and elicited by GaGM. Both activities induced were lower in mice primed with trehalose mono- or dimycolate from R. aurantiacus (GaTMM, GaTDM) or TDM from Nocardia rubra than in GaGM-primed mice. Time course study showed that the maximum activity of each interferon (alpha, beta, or gamma) was observed at different stages after LPS administration; IFN-alpha, IFN-beta, and IFN-gamma appeared 3, 2, and 6 hours most abundantly after LPS administration, respectively.
对一种来自独特嗜冷抗酸细菌——橙色红球菌的新型霉菌酰糖脂海藻糖2,3,6'-三霉菌酸酯（GaGM）的免疫调节活性进行了研究。给ICR小鼠静脉内（i.v.）或腹腔内（i.p.）注射含GaGM的脂质体（300微克/小鼠）进行致敏，2周后给小鼠静脉注射溶解于生理盐水的LPS（25微克/小鼠）。注射LPS两小时后，小鼠血清中显著诱导出干扰素（IFN）和肿瘤坏死因子（TNF）。IFN和TNF活性的增加与用GaGM致敏的小鼠脾脏中的肉芽肿形成大致平行。然而，在用GaGM致敏但未用LPS激发的小鼠中，或在用GaGM致敏并用GaGM激发的小鼠中，均未诱导出IFN和TNF。用来自橙色红球菌的海藻糖单霉菌酸酯或双霉菌酸酯（GaTMM、GaTDM）或来自红色诺卡氏菌的TDM致敏的小鼠中，诱导的两种活性均低于用GaGM致敏的小鼠。时间进程研究表明，在给予LPS后的不同阶段观察到每种干扰素（α、β或γ）的最大活性；给予LPS后，IFN-α、IFN-β和IFN-γ分别在3、2和6小时出现得最为丰富。
