Isoda H, Shinmoto H, Matsumura M, Nakahara T
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Cytotechnology. 1995;19(1):79-88. doi: 10.1007/BF00749758.
A novel type of succinoyl trehalose lipid (STL-1) prepared from n-hexadecane-culture of Rhodococcus erythropolis SD-74 markedly inhibited the growth of a human monocytoid leukemic cell line, U937, and induced its morphological alteration along a monocyte-macrophage lineage. STL-1 markedly increased differention-associated characteristics in macrophage, such as nitroblue tetrazolium reducing ability, appearance of Fc receptor, phagocytic activities in U937. Furthermore, U937 cells, which were activated with STL-1 exhibited cytotoxic activity against human lung carcinoma cell line A549. However, STL-1 did not affect growth of a normal human fetal lung cell line TIG-1. The individual components of STL-1, neither sugar moiety nor fatty acids in the free form, were effective at inducing the differentiation of U937 cell. From these results, we concluded that STL-1 has low cytotoxicity against normal human cells and the ester molecule itself is responsible for the activity of inducing differentiation of human monocytoid leukemic cell line U937 into monocyte-macrophage which results in the stimulation of the production of some cytotoxic substances.
从正十六烷培养的红平红球菌SD - 74制备的一种新型琥珀酰海藻糖脂（STL - 1）显著抑制人单核细胞白血病细胞系U937的生长，并诱导其沿单核细胞 - 巨噬细胞谱系发生形态改变。STL - 1显著增强了巨噬细胞中与分化相关的特性，如硝基蓝四氮唑还原能力、Fc受体的出现、U937中的吞噬活性。此外，用STL - 1激活的U937细胞对人肺癌细胞系A549表现出细胞毒活性。然而，STL - 1不影响正常人类胎儿肺细胞系TIG - 1的生长。STL - 1的各个组分，无论是游离形式的糖部分还是脂肪酸，都不能有效诱导U937细胞分化。从这些结果来看，我们得出结论，STL - 1对正常人类细胞具有低细胞毒性，并且酯分子本身负责诱导人单核细胞白血病细胞系U937分化为单核细胞 - 巨噬细胞的活性，这导致了一些细胞毒性物质产生的刺激。
