Pataky D M, Curtis S B, Buchan A M
Department of Physiology, University of British Columbia, Vancouver, Canada.
Neuroscience. 1990;36(1):247-54. doi: 10.1016/0306-4522(90)90366-c.
Immunocytochemical double and triple staining techniques were employed on whole mounts of the submucosal plexus from normal Wistar and non-diabetic BB rat jejunum and ileum, to determine the patterns of co-localization of vasoactive intestinal polypeptide-, peptide histidine-isoleucine-, somatostatin-, neuropeptide Y-, calcitonin gene-related peptide-, substance P-, and galanin-immunoreactive nerves. Neuropeptide Y immunoreactivity was found in 38% of submucosal plexus neurons, within the same neuronal elements as vasoactive intestinal polypeptide immunoreactivity (39% of submucosal plexus neurons) and peptide histidine-isoleucine immunoreactivity. A small population (1% of submucosal plexus neurons) containing vasoactive intestinal polypeptide- and peptide histide isoleucine-like immunoreactivity without NPY-like immunoreactivity was also observed. A significant population of fibers containing vasoactive intestinal polypeptide and galanin immunoreactivity were observed in the mucosa and submucosa, although no cell bodies were detected which contained both neuropeptides. Galanin-like immunoreactivity was seen in a small (2% of submucosal plexus neurons) population, not co-localized with any of the other neuropeptides examined. All somatostatin-immunoreactive neuronal elements (18% of submucosal plexus neurons) contained calcitonin gene-related peptide immunoreactivity, just over half of which also contained substance P immunoreactivity. An additional 25% of submucosal plexus neurons contained calcitonin gene-related peptide- without somatostatin-like immunoreactivity and 28% of submucosal plexus neurons contained substance P without somatostatin-like immunoreactivity. Some degree of co-localization was seen between calcitonin gene-related peptide- and substance P-like immunoreactivity, however, this could not be directly quantified.(ABSTRACT TRUNCATED AT 250 WORDS)
采用免疫细胞化学双重和三重染色技术，对正常Wistar大鼠和非糖尿病BB大鼠空肠和回肠黏膜下神经丛的整装标本进行研究，以确定血管活性肠肽、组异肽、生长抑素、神经肽Y、降钙素基因相关肽、P物质和甘丙肽免疫反应性神经的共定位模式。在38%的黏膜下神经丛神经元中发现神经肽Y免疫反应性，与血管活性肠肽免疫反应性（39%的黏膜下神经丛神经元）和组异肽免疫反应性存在于相同的神经元成分中。还观察到一小部分（1%的黏膜下神经丛神经元）含有血管活性肠肽和组异肽样免疫反应性但无神经肽Y样免疫反应性。在黏膜和黏膜下层观察到大量含有血管活性肠肽和甘丙肽免疫反应性的纤维，尽管未检测到同时含有这两种神经肽的细胞体。在一小部分（2%的黏膜下神经丛神经元）中可见甘丙肽样免疫反应性，与所检测的其他神经肽均无共定位。所有生长抑素免疫反应性神经元成分（18%的黏膜下神经丛神经元）都含有降钙素基因相关肽免疫反应性，其中略超过一半还含有P物质免疫反应性。另外25%的黏膜下神经丛神经元含有降钙素基因相关肽但无生长抑素样免疫反应性，28%的黏膜下神经丛神经元含有P物质但无生长抑素样免疫反应性。降钙素基因相关肽和P物质样免疫反应性之间存在一定程度的共定位，然而，这无法直接定量。（摘要截短于250字）
