Ekblad E, Ekman R, Håkanson R, Sundler F
Department of Histology, University of Lund, Sweden.
Neuroscience. 1988 Nov;27(2):655-74. doi: 10.1016/0306-4522(88)90296-5.
The distribution, origin and projections of nerve fibers containing vasoactive intestinal peptide, substance P, neuropeptide Y, galanin, gastrin-releasing peptide, calcitonin gene-related peptide, somatostatin or enkephalin were studied in the midcolon of the rat by immunocytochemistry and immunochemistry. Most of these nerve fibers had an intramural origin as was established by extrinsic denervation (serving of mesenterial nerves). Extrinsic denervation eliminated neuropeptide Y-containing fibers of presumably sympathetic origin together with sensory nerve fibers containing both substance P and calcitonin gene-related peptide. Co-existence of two peptides in the same neuron was studied by double immunostaining. This revealed co-existence of neuropeptide Y and vasoactive intestinal peptide in one population of intramural neurons; an additional population of intramural neurons was found to contain vasoactive intestinal peptide but not neuropeptide Y. All somatostatin-containing neurons in the submucous ganglia were found to harbor calcitonin gene-related peptide. A much larger population of submucous neurons containing calcitonin gene-related but not somatostatin was also detected. Some perivascular calcitonin gene-related peptide-containing nerve fibers (of intrinsic origin) harbored vasoactive intestinal peptide while others (of extrinsic origin) harbored substance P. The polarities and projections of the various peptide-containing intramural neurons in the transverse colon were studied by analysing the loss of nerve fibers upon local disruption of enteric nervous pathways (myectomy or intestinal clamping). Myenteric neurons containing vasoactive intestinal peptide, galanin, gastrin-releasing peptide, calcitonin gene-related peptide, somatostatin or vasoactive intestinal peptide/neuropeptide Y gave off 5-10-mm-long descending projections while those containing substance P or enkephalin issued approx. 5-mm-long ascending projections. Submucous neurons containing calcitonin gene-related peptide, somatostatin/calcitonin gene-related peptide or gastrin-releasing peptide issued both ascending (2-6 mm) and descending (2-6 mm) projections, those containing vasoactive intestinal peptide issued ascending (approx. 2 mm) projections, while those containing galanin or vasoactive intestinal peptide/neuropeptide Y lacked demonstrable oro-anal projections. Enkephalin-containing fibers could not be detected in the mucosa and the mucosal substance P-containing nerve fibers were too few to enable us to delineate their projections.
采用免疫细胞化学和免疫化学方法，研究了大鼠中结肠内含有血管活性肠肽、P物质、神经肽Y、甘丙肽、胃泌素释放肽、降钙素基因相关肽、生长抑素或脑啡肽的神经纤维的分布、起源及投射。通过去神经支配（切断肠系膜神经）证实，这些神经纤维大多起源于壁内。去神经支配消除了可能起源于交感神经的含神经肽Y的纤维以及同时含有P物质和降钙素基因相关肽的感觉神经纤维。通过双重免疫染色研究了同一神经元中两种肽的共存情况。结果显示，壁内神经元的一个群体中神经肽Y和血管活性肠肽共存；还发现另一个壁内神经元群体含有血管活性肠肽但不含神经肽Y。发现黏膜下神经节中所有含生长抑素的神经元都含有降钙素基因相关肽。还检测到大量含有降钙素基因相关肽但不含生长抑素的黏膜下神经元。一些含降钙素基因相关肽的血管周围神经纤维（起源于固有神经）含有血管活性肠肽，而其他一些（起源于外来神经）含有P物质。通过分析肠道神经通路局部破坏（肌切除术或肠钳夹术）后神经纤维的损失情况，研究了横结肠中各种含肽壁内神经元的极性和投射。含有血管活性肠肽、甘丙肽、胃泌素释放肽、降钙素基因相关肽、生长抑素或血管活性肠肽/神经肽Y的肌间神经元发出5 - 10毫米长的下行投射，而含有P物质或脑啡肽的肌间神经元发出约5毫米长 的上行投射。含有降钙素基因相关肽、生长抑素/降钙素基因相关肽或胃泌素释放肽的黏膜下神经元发出上行（2 - 6毫米）和下行（2 - 6毫米）投射，含有血管活性肠肽的黏膜下神经元发出上行（约2毫米）投射，而含有甘丙肽或血管活性肠肽/神经肽Y的黏膜下神经元没有明显的口 - 肛投射。在黏膜中未检测到含脑啡肽的纤维，含P物质的黏膜神经纤维太少，无法确定其投射。
