Iwadare T, Harada E, Yoshino S, Arai T
Research Laboratory, Sakura Finetechnical Co., Tokyo, Japan.
Stain Technol. 1990;65(4):205-9. doi: 10.3109/10520299009108071.
Development of a resin-dissolving solution for use at low alkali concentrations is described. Crown ether dissolved in dimethyl-sulfoxide produces a superbasic alkoxide anion. A five minute treatment resulted in complete resin removal from kidney biopsy specimens embedded in Epon 812. Specimens were well stained by Loeffler's methylene blue. Periodic acid-methenamine silver and Giemsa stains yielded good results. Application of PAS reaction and subsequent hematoxylin counterstaining was practicable for diagnosis.
本文描述了一种用于低碱浓度的树脂溶解溶液的开发。溶解在二甲基亚砜中的冠醚会产生超碱性醇盐阴离子。五分钟的处理可使包埋在Epon 812中的肾活检标本中的树脂完全去除。标本用吕弗勒亚甲蓝染色良好。过碘酸-六胺银染色和吉姆萨染色效果良好。应用PAS反应并随后进行苏木精复染对诊断可行。
