Iwadare T, Arai T
Research Laboratory, Sakura Finetechnical Co., Tokyo, Japan.
Biotech Histochem. 1995 Mar;70(2):53-6. doi: 10.3109/10520299509108317.
Staining of etched sections for light microscopy is described. Azan staining was successful after treatment with potassium dichromate and the use of concentrated dye solutions. To remove osmium for hematoxylin-eosin staining, removal by reduction with ferrocene was used instead of oxidation. Highly selective differentiation after hematoxylin staining was achieved using p-toluenesulfonic acid-DMSO. To enhance eosin staining, a 2-bromoethylamine link between eosin and the tissue was used. Ferrocene also facilitated counterstaining of nuclei with hematoxylin after the PAS reaction. Periodic acid-methenamine silver staining was carried out without modification.
描述了用于光学显微镜检查的蚀刻切片染色方法。用重铬酸钾处理并使用浓染料溶液后，偶氮染色成功。为了去除苏木精 - 伊红染色中的锇，采用二茂铁还原去除法而非氧化法。使用对甲苯磺酸 - 二甲基亚砜实现了苏木精染色后的高选择性分化。为增强伊红染色效果，在伊红与组织之间使用了2 - 溴乙胺连接。二茂铁还促进了过碘酸 - 亚甲胺银反应后细胞核用苏木精的复染。过碘酸 - 亚甲胺银染色未作修改直接进行。
