Pfister C, Glede G, Schön E
Institut für Anatomie des Bereiches Medizin (Charité), Humboldt-Universität zu Berlin.
Acta Histochem. 1990;88(2):109-13.
From children with growth retardation due to enteric disease, duodenal jejunal biopsy material was investigated. Using a modified Feulgen-reaction--acridine orange staining after acid hydrolysis according to Fukuda et al. (1986)--different DNA forms could be demonstrated in nuclei of cells of the lamina epithelialis mucosae by fluorescence microscopy. These clear cut forms of DNA by different fluorescence colours were only found in altered mucosa. The findings were discussed with regard to the possibility of mitotic disorders and cancerogenesis.
对患有肠道疾病所致生长发育迟缓的儿童的十二指肠空肠活检材料进行了研究。根据福田等人（1986年）的方法，采用改良的福尔根反应——酸水解后吖啶橙染色，通过荧光显微镜可在黏膜上皮层细胞的细胞核中显示出不同的DNA形态。这些通过不同荧光颜色清晰呈现的DNA形态仅在病变黏膜中发现。针对有丝分裂紊乱和癌变的可能性对这些发现进行了讨论。
