Larsimont D, Kiss R, de Launoit Y, Melamed M R
Department of Pathology, Institut J. Bordet, Faculty of Medicine, Universite Libre de Bruxelles, Belgium.
Am J Clin Pathol. 1990 Oct;94(4):378-83. doi: 10.1093/ajcp/94.4.378.
The authors analyzed several cytomorphonuclear parameters related to chromatin distribution and DNA ploidy in typical and atypical carcinoids and in small cell lung cancers. Nuclear measurements and analysis were performed with a SAMBA 200 (TITN, Grenoble, France) cell image processor with software allowing the discrimination of parameters computed on cytospin preparations of Feulgen-stained nuclei extracted from deparaffinized tumor tissues. The authors' results indicate a significant increase in DNA content--assessed by integrated optical density (IOD)--from typical carcinoids to small cell lung carcinomas, with atypical carcinoids showing an intermediate value. Parameters related to hyperchromatism (short and long run length and variance of optical density) also characterize the atypical carcinoids as being intermediate between typical carcinoids and small cell lung cancers. The systematic measurement of these cytomorphonuclear parameters seems to define an objective, reproducible "scale" of differentiation that helps to define the atypical carcinoid and may be of value in establishing cytologic criteria for differential diagnosis.
作者分析了典型类癌、非典型类癌和小细胞肺癌中与染色质分布及DNA倍体相关的几个细胞形态核参数。使用SAMBA 200（法国格勒诺布尔的TITN公司）细胞图像处理器进行核测量与分析，该处理器配备的软件能够区分从脱蜡肿瘤组织中提取的福尔根染色细胞核的细胞涂片制备物上计算出的参数。作者的结果表明，通过积分光密度（IOD）评估的DNA含量从典型类癌到小细胞肺癌显著增加，非典型类癌呈现中间值。与核深染相关的参数（短游程长度、长游程长度和光密度方差）也表明非典型类癌处于典型类癌和小细胞肺癌之间的中间状态。对这些细胞形态核参数进行系统测量似乎能定义一个客观、可重复的分化“尺度”，这有助于界定非典型类癌，并且在建立鉴别诊断的细胞学标准方面可能具有价值。
