Ler Siok Ghee, Lee Fook Kay, Gopalakrishnakone P
Venom and Toxin Research Programme, Yong Loo Lin School of Medicine, National University of Singapore, 4 Medical Drive, Singapore 117597, Singapore.
J Chromatogr A. 2006 Nov 10;1133(1-2):1-12. doi: 10.1016/j.chroma.2006.08.078. Epub 2006 Sep 22.
An overview of the different detection methods available for ricin, staphylococcal enterotoxin B (SEB) and T-2 toxin is presented here. These toxins are potential biological warfare agents (BWA). The aim of this review is not to cover all the papers that had been published but rather to give an overall picture of the trend in the detection methodologies for potential biological warfare agents as we do see the emerging threats from these three toxins. The advantages and disadvantages of each methodology as well as the detection limit will be reviewed. It seems that mass spectrometry has created a niche for analysis of proteinaceous toxins, ricin and SEB as well as molecular toxin, T-2 toxin given its high sensitivity, high selectivity, high specificity and capability to identify and quantify unknown agents simultaneously in a short time frame. But its main drawbacks are its sophisticated instrumentation and its high cost. Improvised immunoassay may be an alternative.
本文概述了可用于检测蓖麻毒素、葡萄球菌肠毒素B（SEB）和T-2毒素的不同检测方法。这些毒素是潜在的生物战剂（BWA）。本综述的目的不是涵盖所有已发表的论文，而是鉴于我们确实看到了这三种毒素带来的新威胁，对潜在生物战剂检测方法的趋势给出一个总体情况。将对每种方法的优缺点以及检测限进行综述。鉴于其高灵敏度、高选择性、高特异性以及能够在短时间内同时识别和定量未知试剂的能力，质谱似乎在蛋白质毒素、蓖麻毒素和SEB以及分子毒素T-2毒素的分析方面占据了一席之地。但其主要缺点是仪器复杂且成本高昂。简易免疫测定可能是一种替代方法。
