Qie Zhiwei, Shi Jinmiao, Yan Wenliang, Gao Zichen, Meng Wu, Xiao Rui, Wang Shengqi
Beijing Institute of Radiation Medicine Beijing 100850 PR China
Center for Diseases Prevention and Control of Rocket Force China.
RSC Adv. 2019 Nov 26;9(66):38697-38702. doi: 10.1039/c9ra06689f. eCollection 2019 Nov 25.
A quantum dot bead based immunochromatographic assay (QB-ICA) system was established for T-2 toxin (T-2), which widely occurs in agriculture and could be used as a potential biological warfare agent. After optimization, the dynamic linear detection range of T-2 calculated from a calibration curve was from 0.12 to 0.67 ng mL and the limit of detection (LOD) was 0.08 ng mL, which is lower than those of the ICA based on colloidal gold nanoparticles or a fluorescent material or an antibody-based biochip in other reports. The performance and practicability of the established ICA system were validated with a commercial ELISA kit and the two methods were comparable. The proposed QB-ICA for T-2 could be an alternative for rapid, sensitive, and quantitative on-site detection of this toxin in biosafety monitoring in agriculture and for susceptibility testing of the potential release of this biological warfare agent.
建立了一种基于量子点微球的免疫层析检测（QB-ICA）系统用于检测T-2毒素，T-2毒素广泛存在于农业领域，且可能被用作潜在的生物战剂。经过优化，根据校准曲线计算得出的T-2动态线性检测范围为0.12至0.67 ng/mL，检测限（LOD）为0.08 ng/mL，低于其他报告中基于胶体金纳米颗粒、荧光材料或抗体生物芯片的免疫层析检测方法。使用商用ELISA试剂盒对所建立的免疫层析检测系统的性能和实用性进行了验证，两种方法具有可比性。所提出的用于检测T-2毒素的QB-ICA可作为一种替代方法，用于农业生物安全监测中该毒素的快速、灵敏和定量现场检测，以及该生物战剂潜在释放的易感性测试。
