猪传染性胃肠炎（TGE）：猪睾丸细胞培养单层的年龄对TGE病毒空斑试验的影响
Transmissible gastroenteritis (TGE) of swine: effect of age of swine testes cells culture monolayers on plaque assays of TGE virus.
作者信息
Stark S L, Fernelius A L, Booth G D, Lambert G
出版信息
Can J Comp Med. 1975 Oct;39(4):466-8.
Abstract
A continuous line of swine testes cell culture monolayers was infected at various ages with both cell culture-adapted transmissible gastroenteritis (TGE) virus and tissue infected with TGE virus. Both produced increasing numbers of plaques as the cell monolayers aged from two to five days. Therefore, allowing the swine testes cell monolayer to age five to six days before inoculation should increase the likelihood of detecting TGE virus by plaque assay.
摘要
用适应细胞培养的传染性胃肠炎（TGE）病毒和感染TGE病毒的组织在不同年龄感染连续的猪睾丸细胞培养单层。随着细胞单层从2天龄到5天龄，两者产生的蚀斑数量都在增加。因此，在接种前让猪睾丸细胞单层达到5至6天龄应会增加通过蚀斑测定法检测TGE病毒的可能性。
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