Woods Roger D, Wesley Ronald D
National Animal Disease Center, USDA-ARS, P.O. Box 70, 50010 Ames, Iowa.
J Tissue Cult Methods. 1988;11(2):95-100. doi: 10.1007/BF01404139.
Techniques are described for the growth and characterization of some mammalian coronaviruses. Because of the fastidious nature of their growth requirements, most will replicate only in cells derived from the natural host or a closely related species. Fetal cat cells are used to grow FIPV, and porcine cells are used to grow TGEV and HEV. However, CCV will replicate in both feline and canine cells. Although all four of these viruses prefer to replicate in a cell in the stationary phase of growth, FIPV is able to replicate in an actively growing cell. Each virus causes a cytopathic effect in monolayer cell cultures under agar or media 18 to 72 h postinfection. Primary isolation of each virus from field specimens is difficult, although most can usually be isolated after 1 to 3 blind passages in the cell culture.
描述了一些哺乳动物冠状病毒的培养及特性鉴定技术。由于它们对生长条件要求苛刻，大多数病毒仅在源自天然宿主或密切相关物种的细胞中复制。胎猫细胞用于培养猫传染性腹膜炎病毒（FIPV），猪细胞用于培养猪传染性胃肠炎病毒（TGEV）和猪戊型肝炎病毒（HEV）。然而，犬冠状病毒（CCV）能在猫和犬的细胞中复制。尽管这四种病毒都倾向于在处于生长静止期的细胞中复制，但FIPV能够在活跃生长的细胞中复制。在感染后18至72小时，每种病毒在琼脂或培养基覆盖下的单层细胞培养物中都会引起细胞病变效应。从野外样本中初次分离每种病毒都很困难，但大多数病毒通常在细胞培养中经过1至3次盲传后能够分离出来。
