含有2-乙酰氨基-4-O-[(S)-1-羧乙基]-2-脱氧-D-葡萄糖的大肠杆菌O150 O-多糖的结构

Structure of the O-polysaccharide of Escherichia coli O150 containing 2-acetamido-4-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose.

作者信息

Perepelov Andrei V, Han Weiqing, Senchenkova Sof'ya N, Shevelev Sergei D, Shashkov Alexander S, Feng Lu, Liu Yanqun, Knirel Yuriy A, Wang Lei

机构信息

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.

出版信息

Carbohydr Res. 2007 Feb 26;342(3-4):648-52. doi: 10.1016/j.carres.2006.08.017. Epub 2006 Sep 25.

DOI:10.1016/j.carres.2006.08.017

PMID:16997291

Abstract

An acidic O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Escherichia coli O150 and studied by sugar and methylation analyses, triflic acid solvolysis, Smith degradation, (1)H and (13)C NMR spectroscopy, including 2D ROESY, (1)H,(13)C HSQC, HMQC-TOCSY, and HMBC experiments. The polysaccharide was found to contain a regioisomer of N-acetylisomuramic acid, 2-acetamido-4-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose [d-GlcNAc4(Slac)]. The structure of its hexasaccharide repeating unit was established.

摘要

通过对大肠杆菌O150脂多糖进行温和酸降解得到一种酸性O-多糖，并通过糖分析、甲基化分析、三氟甲磺酸醇解、史密斯降解、¹H和¹³C NMR光谱（包括二维ROESY、¹H,¹³C HSQC、HMQC-TOCSY和HMBC实验）对其进行了研究。发现该多糖含有N-乙酰异muramic酸的区域异构体，即2-乙酰氨基-4-O-[(S)-1-羧乙基]-2-脱氧-D-葡萄糖 [d-GlcNAc4(Slac)]。确定了其六糖重复单元的结构。