水生拉恩菌95 U003脂多糖O-特异性多糖的结构

Structure of the O-specific polysaccharide of the lipopolysaccharide of Rahnella aquatilis 95 U003.

作者信息

Zdorovenko Evelina L, Varbanets Lyudmyla D, Zatonsky George V, Kachala Vadim V, Zdorovenko Galina M, Shashkov Alexander S, Knirel Yuriy A

机构信息

N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.

出版信息

Carbohydr Res. 2008 Sep 22;343(14):2494-7. doi: 10.1016/j.carres.2008.07.001. Epub 2008 Jul 9.

DOI:10.1016/j.carres.2008.07.001

PMID:18674754

Abstract

The O-polysaccharide of Rahnella aquatilis 95 U003 was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses, Smith degradation and (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC and HMQC-TOCSY experiments. The O-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure: [carbohydrate structure: see text]

摘要

通过对水生拉恩菌95 U003的脂多糖进行温和酸降解获得了其O-多糖，并通过糖分析、甲基化分析、史密斯降解以及（1）H和（13）C NMR光谱进行研究，包括二维（1）H，（1）H COSY、TOCSY、ROESY、H检测（1）H，（13）C HSQC和HMQC-TOCSY实验。发现该O-多糖具有以下结构的分支六糖重复单元：[碳水化合物结构：见正文]