Staszyk C, Gasse H
Institute of Anatomy, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany.
Res Vet Sci. 2007 Apr;82(2):150-7. doi: 10.1016/j.rvsc.2006.07.003. Epub 2006 Sep 25.
Fibroblasts and cementoblasts in the periodontal ligament (PDL) of equine cheek teeth were harvested, and monocultures were obtained by means of a "selective detachment" procedure. Cells were characterized by morphological criteria and by immunostaining for vimentin, FVIII, pan-cytokeratin, smooth muscle actin, and pro-collagen. Cementogenic potential of the cells was determined by immunostaining for osteopontin and by histochemical detection of alkaline phosphatase. Equine periodontal fibroblasts (EPF) were spindle-shaped and polygonal. Equine dental cementoblasts (EDC) grew in cobblestone-like clusters. Both EPF and EDC stained positive for vimentin. Only EPF contained smooth muscle actin, pro-collagen, and alkaline phosphatase. Few EDC stained positive for osteopontin. The phenotypes of EPF and EDC and their specific expression of proteins corresponded to PDL fibroblasts and dental cementoblasts of other species. These results indicate the potential use of EPF and EDC in an adequate in vitro model of equine cementogenesis and equine periodontal remodeling.
采集马颊齿牙周膜（PDL）中的成纤维细胞和牙骨质细胞，并通过“选择性分离”程序获得单一培养物。通过形态学标准以及波形蛋白、FVIII、泛细胞角蛋白、平滑肌肌动蛋白和前胶原的免疫染色对细胞进行表征。通过骨桥蛋白的免疫染色和碱性磷酸酶的组织化学检测来确定细胞的牙骨质生成潜力。马牙周成纤维细胞（EPF）呈纺锤形和多边形。马牙骨质细胞（EDC）以鹅卵石样簇状生长。EPF和EDC的波形蛋白染色均呈阳性。只有EPF含有平滑肌肌动蛋白、前胶原和碱性磷酸酶。少数EDC的骨桥蛋白染色呈阳性。EPF和EDC的表型及其蛋白质的特异性表达与其他物种的PDL成纤维细胞和牙骨质细胞一致。这些结果表明，EPF和EDC在合适的马牙骨质生成和马牙周重塑体外模型中具有潜在用途。
