Heilen Laura Beate, Roßgardt Jessica, Dern-Wieloch Jutta, Vogelsberg Jörg, Staszyk Carsten
Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University, Giessen, Germany.
Front Vet Sci. 2023 Mar 10;10:1116671. doi: 10.3389/fvets.2023.1116671. eCollection 2023.
The lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling.
In this study, we isolated and cultivated MSCs (medicinal signaling cells) from dental pulp, PDL and retrobulbar fat of four horses. Subsequently, we analyzed them by flow cytometry and immunohistochemistry to determine and compare their characteristics. In addition, we localized these cells within the tissue structure immunohistochemistry of histological sections. For these analyses, several surface markers were applied.
The described method illustrates a feasible approach to isolate and cultivate MSCs from equine dental pulp and PDL. In the flow cytometry a vast majority of cultivated cells were positive for CD90 and CD40 and negative for CD11a/18, CD45, CD105 and MHCII suggesting that these cells feature characteristics of MSCs. Immunohistochemistry of histological pulp and PDL sections showed the localization of CD90 positive cells especially in the perivascular region and the subodontoblastic layer.
Our findings indicate that the isolation and cultivation of MSCs from equine dental pulp and PDL is feasible although an elaborate and complicated harvesting protocol is required. MSCs isolated from dental pulp and PDL are regarded as candidates for new therapeutical approaches in equine dental medicine like regeneration of periodontal lesions, enhancement of periodontal re-attachment after dental replantation and stimulation of pulp-obliteration and apexification in combination with endodontic therapies.
马牙的终生萌出对牙髓和牙周韧带（PDL）提出了很高的要求。牙髓和牙周韧带中的细胞似乎在这种重塑过程中起关键作用。
在本研究中，我们从四匹马的牙髓、牙周韧带和球后脂肪中分离并培养了间充质干细胞（医学信号细胞）。随后，我们通过流式细胞术和免疫组织化学对它们进行分析，以确定并比较它们的特征。此外，我们通过组织学切片的免疫组织化学将这些细胞定位在组织结构内。对于这些分析，应用了几种表面标志物。
所描述的方法说明了一种从马牙髓和牙周韧带中分离并培养间充质干细胞的可行方法。在流式细胞术中，绝大多数培养的细胞CD90和CD40呈阳性，而CD11a/18、CD45、CD105和MHCII呈阴性，这表明这些细胞具有间充质干细胞的特征。牙髓和牙周韧带组织学切片的免疫组织化学显示CD90阳性细胞尤其定位在血管周围区域和成牙本质细胞下层。
我们的研究结果表明，从马牙髓和牙周韧带中分离并培养间充质干细胞是可行的，尽管需要精心且复杂的采集方案。从牙髓和牙周韧带中分离出的间充质干细胞被视为马牙科医学新治疗方法的候选者，如牙周病变的再生、牙齿再植后牙周再附着的增强以及与牙髓治疗相结合的牙髓闭锁和根尖形成的刺激。
