长角血蜱谷胱甘肽 S-转移酶的交叉免疫可降低实验性璃眼蜱（硬蜱属）的侵染。
Cross immunity with Haemaphysalis longicornis glutathione S-transferase reduces an experimental Rhipicephalus (Boophilus) microplus infestation.
机构信息
Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Avenida Bento Gonçalves, 9500 Prédio 43421, Porto Alegre, RS 91501-970, Brazil.
出版信息
Exp Parasitol. 2011 Jan;127(1):113-8. doi: 10.1016/j.exppara.2010.07.001. Epub 2010 Jul 7.
Recombinant Glutathione S-transferase of Haemaphysalis longicornis (rGST-Hl) was expressed in Escherichia coli, purified by affinity chromatography and used in the immunization of cattle. Western blot analysis showed positive antibody response in cattle immunized with rGST-Hl. The tests also showed that immunized bovine sera recognize native Rhipicephalus microplus proteins in different tissue extracts. Furthermore, the vaccine potential of rGST-Hl was investigated against infestation of Hereford cattle by R. microplus. Vaccination of cattle with rGST-Hl conferred partial cross-protection immunity against R. microplus. Considering the effect on number of engorged ticks, egg laying capacity and egg fertility, the overall efficacy of vaccination was of 57%, as compared with control group.
重组长角血蜱谷胱甘肽 S-转移酶（rGST-Hl）在大肠杆菌中表达，通过亲和层析进行纯化，并用于牛的免疫接种。Western blot 分析显示，rGST-Hl 免疫接种的牛产生了阳性抗体反应。这些试验还表明，免疫牛血清可识别不同组织提取物中的原生 Rhipicephalus microplus 蛋白。此外，还研究了 rGST-Hl 对 Hereford 牛感染 R. microplus 的疫苗潜力。用 rGST-Hl 对牛进行疫苗接种可赋予针对 R. microplus 的部分交叉保护免疫。与对照组相比，在考虑对饱血蜱数量、产卵能力和卵育性的影响时，疫苗接种的总体效果为 57%。
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