Reipa Vytas, Hackley Vincent A, Tona Alessandro, Heo Min Beom, Lee Ye Ryeong, Lee Tae Geol, Johnston-Peck Aaron, Cho Tae Joon
Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Division of Biomedical Metrology, Korea Research Institute of Standards and Science, Daejeon 34113, Republic of Korea.
Nanomaterials (Basel). 2025 Jan 7;15(2):79. doi: 10.3390/nano15020079.
The safety screening of manufactured nanomaterials (MNMs) is essential for their adoption by consumers and the marketplace. Lately, animal-based testing has been replaced by mechanistically informative in vitro assays due to the requirements of regulatory agencies. Cell viability assays are widely employed for manufactured nanomaterial hazard screening as a first-tier approach. Critical parts of such assays are positive and negative controls that serve as measurement benchmarks. We present the cellular viability and corresponding particle characterization obtained with eight different cell lines that were exposed to Au-PEI and Au-PEG-COOH nanoparticles. We showed that polyethyleneimine- and carboxylate-polyethylene-glycol-conjugated gold nanoparticles (AuPEI and Au-PEG-COOH) qualified for positive and negative controls in the in vitro cell viability assays used for MNM toxicological screening.
人造纳米材料（MNMs）的安全筛选对于消费者和市场采用这些材料至关重要。最近，由于监管机构的要求，基于动物的测试已被具有机理信息的体外试验所取代。细胞活力测定作为一种一线方法被广泛用于人造纳米材料的危害筛选。此类测定的关键部分是用作测量基准的阳性和阴性对照。我们展示了用八种不同细胞系暴露于金-聚乙烯亚胺（Au-PEI）和金-聚乙二醇-羧基（Au-PEG-COOH）纳米颗粒后获得的细胞活力及相应的颗粒表征。我们表明，聚乙烯亚胺和羧基化聚乙二醇共轭金纳米颗粒（AuPEI和Au-PEG-COOH）符合用于MNM毒理学筛选的体外细胞活力测定中的阳性和阴性对照标准。
