Sadiq Zubi, Safiabadi Tali Seyed Hamid, Mansouri Maryam, Jahanshahi-Anbuhi Sana
Department of Chemical and Materials Engineering, Gina Cody School of Engineering and Computer Science, Concordia University Montréal Québec Canada
Nanoscale Adv. 2025 Mar 20;7(10):2967-2978. doi: 10.1039/d5na00082c. eCollection 2025 May 13.
Point-of-care (POC) devices provide on-site disease diagnosis, particularly in resource-limited settings. Despite considerable progress in POC testing, the availability of commercial devices remains limited, primarily due to challenges in detection sensitivity and portability. Furthermore, advancements in existing POC devices are essential to better meet the needs of end-users. Herein, we present a colorimetric dual-functional tablet sensor using dextran-gold nanoparticles (dAuNPs) to detect and quantify uric acid and glucose levels in urine. Our tablet sensor combines the plasmonic and nanozyme properties of dAuNPs, resulting in highly sensitive detection of both biomarkers. Interestingly, we fabricated the nanogold tablet directly from the dAuNP solution without the addition of any external stabilizer or tablet-forming reagent, thus naming it a direct tablet. An enzyme-free approach was employed for uric acid detection, providing a wide detection range of 0.00187-7.8 mM and a low detection limit of 0.0037 mM, attributed to the hydrogen bonding between dextran and uric acid. On the other hand, the unique nanozyme properties of dAuNPs exhibited exclusive POx-mimetic activity for glucose detection ( = 0.106 mM and = 369.72 mM min), with a lower detection limit of 0.625 mM. Our dual-functional tablet offers exceptional substrate selectivity for the colorimetric-chromogenic assay of both uric acid and glucose. This dual-functionality not only provides a highly sensitive, selective, and cost-effective detection strategy for resource-limited settings but also introduces a new avenue for designing customizable plasmonic-nanozyme nanogold tablet sensors as a powerful tool for rapid diagnosis.
即时检测（POC）设备可进行现场疾病诊断，在资源有限的环境中尤为如此。尽管POC检测取得了长足进展，但商业设备的可用性仍然有限，这主要是由于检测灵敏度和便携性方面存在挑战。此外，改进现有POC设备对于更好地满足终端用户的需求至关重要。在此，我们展示了一种比色双功能平板传感器，该传感器使用葡聚糖-金纳米颗粒（dAuNPs）来检测和定量尿液中的尿酸和葡萄糖水平。我们的平板传感器结合了dAuNPs的等离子体和纳米酶特性，从而实现了对两种生物标志物的高灵敏度检测。有趣的是，我们直接从dAuNP溶液中制备了纳米金平板，无需添加任何外部稳定剂或平板成型试剂，因此将其命名为直接平板。采用无酶方法检测尿酸，检测范围宽，为0.00187 - 7.8 mM，检测限低，为0.0037 mM，这归因于葡聚糖与尿酸之间的氢键。另一方面，dAuNPs独特的纳米酶特性在葡萄糖检测中表现出独特的过氧化物酶模拟活性（米氏常数 = 0.106 mM，最大反应速率 = 369.72 mM min），检测限较低，为0.625 mM。我们的双功能平板为尿酸和葡萄糖的比色显色测定提供了出色的底物选择性。这种双功能性不仅为资源有限的环境提供了一种高度灵敏、选择性强且经济高效的检测策略，还为设计可定制的等离子体-纳米酶纳米金平板传感器开辟了一条新途径，作为快速诊断的有力工具。
