17β-雌二醇对大鼠阴道上皮中角蛋白及其他多肽磷酸化和去磷酸化的调节作用
Modulation of phosphorylation and dephosphorylation of keratin and other polypeptides by estradiol-17 beta in rat vaginal epithelium.
作者信息
Gupta P D, Swarup G, Reddy A G
机构信息
Centre for Cellular and Molecular Biology, Hyderabad, India.
出版信息
FEBS Lett. 1990 Oct 29;273(1-2):135-8. doi: 10.1016/0014-5793(90)81068-y.
Phosphorylation of keratin polypeptides was studied by incubating vaginal tissues (removed from estradiol primed and unprimed 30-day-old rats) with 32Pi. Analysis by SDS-PAGE and autoradiography showed that on treatment with estradiol phosphorylation of 63 and 58 kDa keratin polypeptides increased 3- and 2-fold respectively. Phosphorylation was maximal after 30 min of estradiol priming and decreased thereafter. Phosphorylation of some non-keratin polypeptides (37, 34, 32 and 25 kDa) also showed time dependent variation. The results showed that estradiol can modulate phosphorylation-dephosphorylation of keratins and other polypeptides in rat vaginal epithelial cells.
通过用³²Pᵢ孵育阴道组织（取自经雌二醇预处理和未经预处理的30日龄大鼠）来研究角蛋白多肽的磷酸化。SDS - PAGE和放射自显影分析表明，用雌二醇处理后，63 kDa和58 kDa角蛋白多肽的磷酸化分别增加了3倍和2倍。雌二醇预处理30分钟后磷酸化达到最大值，此后下降。一些非角蛋白多肽（37、34、32和25 kDa）的磷酸化也表现出时间依赖性变化。结果表明，雌二醇可以调节大鼠阴道上皮细胞中角蛋白和其他多肽的磷酸化 - 去磷酸化过程。
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