Gupta P D, Swarup G, Reddy A G
Centre for Cellular and Molecular Biology, Hyderabad, India.
FEBS Lett. 1990 Oct 29;273(1-2):135-8. doi: 10.1016/0014-5793(90)81068-y.
Phosphorylation of keratin polypeptides was studied by incubating vaginal tissues (removed from estradiol primed and unprimed 30-day-old rats) with 32Pi. Analysis by SDS-PAGE and autoradiography showed that on treatment with estradiol phosphorylation of 63 and 58 kDa keratin polypeptides increased 3- and 2-fold respectively. Phosphorylation was maximal after 30 min of estradiol priming and decreased thereafter. Phosphorylation of some non-keratin polypeptides (37, 34, 32 and 25 kDa) also showed time dependent variation. The results showed that estradiol can modulate phosphorylation-dephosphorylation of keratins and other polypeptides in rat vaginal epithelial cells.
通过用³²Pᵢ孵育阴道组织（取自经雌二醇预处理和未经预处理的30日龄大鼠）来研究角蛋白多肽的磷酸化。SDS - PAGE和放射自显影分析表明，用雌二醇处理后，63 kDa和58 kDa角蛋白多肽的磷酸化分别增加了3倍和2倍。雌二醇预处理30分钟后磷酸化达到最大值，此后下降。一些非角蛋白多肽（37、34、32和25 kDa）的磷酸化也表现出时间依赖性变化。结果表明，雌二醇可以调节大鼠阴道上皮细胞中角蛋白和其他多肽的磷酸化 - 去磷酸化过程。
