Gupta P D, Relia S B, Rao S B, Reddy A G
Centre for Cellular and Molecular Biology, Hyderabad, India.
J Steroid Biochem Mol Biol. 1990 Nov 30;37(4):521-7. doi: 10.1016/0960-0760(90)90396-3.
Changes in the calcium levels under the influence of estradiol were investigated in rat vaginal epithelial cells (VEC). After single estradiol injection, the immature rats showed 1.5-fold increase in Ca2+ levels within 15 min when compared to control animals. Progesterone priming brought calcium levels well below control values throughout the experimental period (up to 12 h). Ca2+ levels in serum did not show any appreciable change. Localization of calcium in VEC with electron microscopy showed aggregates of calcium oxalate on the inner nuclear membrane, nucleolus, mitochondria and keratohyaline granules. After 15 min of estradiol priming, maximum electron density was seen on all these cell organelles mentioned above, however, by 30 min the electron density was reduced considerably and did not increase during the experimental period (up to 12 h).
在大鼠阴道上皮细胞（VEC）中研究了雌二醇影响下钙水平的变化。单次注射雌二醇后，与对照动物相比，未成熟大鼠在15分钟内Ca2+水平增加了1.5倍。孕酮预处理使整个实验期间（长达12小时）的钙水平远低于对照值。血清中的Ca2+水平未显示出任何明显变化。用电子显微镜观察VEC中钙的定位，结果显示在内核膜、核仁、线粒体和透明角质颗粒上有草酸钙聚集体。雌二醇预处理15分钟后，在上述所有细胞器上均可见最大电子密度，然而，到30分钟时，电子密度大幅降低，并且在实验期间（长达12小时）未再增加。
