Christensen M M, Keith I, Rhodes P R, Graziano F M, Madsen P O, Bruskewitz R C, Saban R
Urology Section, VA Hospital, Madison, Wisconsin.
J Urol. 1990 Nov;144(5):1293-300. doi: 10.1016/s0022-5347(17)39722-7.
To study the function of mast cells in bladder tissue, guinea pigs were sensitized with ovalbumin by intraperitoneal injections, bladder tissue strips were superfused, and tissue contractile force and histamine release were studied. Upon challenge with ovalbumin, bladder tissue contracted 64 +/- 4% (mean +/- S.E.M.) of the maximum carbachol contraction and released 14.1 +/- 1.6% of the total tissue histamine content. Incubation of sensitized bladder tissue with indomethacin led to an increased force and duration of the contraction while incubation with nordihydroguaiaretic acid combined with pyrilamine reduced histamine release and abolished the contraction. Tissue histamine content was significantly higher in the bladder neck than in the dome, and significantly elevated following sensitization. Histochemical studies of bladder tissue demonstrated mast cell degranulation in antigen challenge experiments. In addition, a group of guinea pigs were sensitized to ovalbumin through bladder instillations. With this model, study of the functional characteristics of bladder mast cells and the acute actions of mast cell products on the bladder microenvironment, should now be feasible.
为研究肥大细胞在膀胱组织中的功能，通过腹腔注射用卵清蛋白使豚鼠致敏，对膀胱组织条进行灌流，并研究组织收缩力和组胺释放情况。用卵清蛋白激发后，膀胱组织收缩达最大卡巴胆碱收缩的64±4%（平均值±标准误），并释放总组织组胺含量的14.1±1.6%。用吲哚美辛孵育致敏膀胱组织导致收缩力增强和收缩持续时间延长，而用去甲二氢愈创木酸联合吡苄明孵育则减少组胺释放并消除收缩。膀胱颈部的组织组胺含量显著高于膀胱顶部，致敏后显著升高。膀胱组织的组织化学研究显示在抗原激发实验中有肥大细胞脱颗粒现象。此外，一组豚鼠通过膀胱灌注使对卵清蛋白致敏。利用该模型，现在应该可以研究膀胱肥大细胞的功能特性以及肥大细胞产物对膀胱微环境的急性作用。
