Krasnopolsky L, Levin R M, Longhurst P A
Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, USA.
Pharmacology. 1995 Feb;50(2):119-27. doi: 10.1159/000139273.
Bladder strips from sensitized guinea pigs respond to ovalbumin challenge with a contraction accompanied by release of histamine, prostaglandin, and leukotriene. Histamine and prostaglandin release occurs preferentially from the bladder mucosa, while leukotrienes are released by the smooth muscle. This study investigated the effects of removal of the bladder mucosa on contractile responses of strips from control and sensitized guinea pigs to electrical stimulation and to contractile agonists. Removal of the mucosa potentiated the contractile response to electrical stimulation at low voltages and pulse durations. The presence of ovalbumin had no effects on the response to electrical stimulation. Removal of the mucosa had no effect on the contractile responses of bladder strips to bethanechol, histamine, KCl, or ovalbumin, although after correction for the decreased strip mass after mucosa removal there was a tendency for the responses to increase. We conclude that the responsiveness of bladder strips from sensitized guinea pigs to ovalbumin challenge results primarily from effects which occur within the smooth muscle.
致敏豚鼠的膀胱条对卵清蛋白激发产生反应，出现收缩并伴有组胺、前列腺素和白三烯的释放。组胺和前列腺素的释放主要发生在膀胱黏膜，而白三烯则由平滑肌释放。本研究调查了去除膀胱黏膜对对照和致敏豚鼠膀胱条对电刺激及收缩性激动剂的收缩反应的影响。去除黏膜增强了在低电压和脉冲持续时间下对电刺激的收缩反应。卵清蛋白的存在对电刺激反应无影响。去除黏膜对膀胱条对氨甲酰甲胆碱、组胺、氯化钾或卵清蛋白的收缩反应无影响，尽管在纠正去除黏膜后条带质量下降后，反应有增加的趋势。我们得出结论，致敏豚鼠膀胱条对卵清蛋白激发的反应性主要源于平滑肌内发生的效应。
