藤壶肌纤维中离子化镁的浓度。
The concentration of ionized magnesium in barnacle muscle fibres.
作者信息
Brinley F J, Scarpa A, Tiffert T
出版信息
J Physiol. 1977 Apr;266(3):545-65. doi: 10.1113/jphysiol.1977.sp011781.
Abstract
- The total Mg in isolated fibres of Balanus aquila was 10-5 m-mole/kg wet wt. 2. The intracellular free Mg was measured by a null point method using Eriochrome Blue as an indicator of free Mg, and internal dialysis with solutions of varying ionized Mg concentrations. The results indicated a free Mg of 6 mM or 4-2 m-mole/kg wet wt. in the intracellular water immediately surrounding the dialysis capillary. 3. The ATP concentration was estimated to be 4-9 m-mole/kg wet wt. 4. A tentative partitioning of Mg among various intracellular constitutents based on present data combined with published work by others is (m-mole/kg wet wt): free, 4-2; MgATP, 4-2; myofibrillar bound, 1; residual (presumably bound to arginine phosphate and phosphate) ca. 1.
摘要
- 黑赭色藤壶分离纤维中的总镁含量为10 - 5毫摩尔/千克湿重。2. 细胞内游离镁通过零点法测量，使用铬蓝作为游离镁的指示剂，并采用不同离子化镁浓度的溶液进行内部透析。结果表明，在透析毛细管周围紧邻的细胞内水中，游离镁为6毫摩尔或4 - 2毫摩尔/千克湿重。3. ATP浓度估计为4 - 9毫摩尔/千克湿重。4. 根据现有数据并结合其他人已发表的研究成果，对镁在各种细胞内成分中的初步分配情况为（毫摩尔/千克湿重）：游离态，4 - 2；MgATP，4 - 2；肌原纤维结合态，1；残余（可能与磷酸精氨酸和磷酸盐结合）约1。
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