Lin Xiaoyan, Tang Min, Zhang Xingdong
School of Materials, Southwest University of Science and Technology, Mianyang 621002, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Aug;23(4):822-5.
Membranes prepared from bovine skin collagen were exposed to 15, 25, 35 KGy gamma-radiation respectively at low temperature. Radiation dose rate of about 22 KGy/h was used. The stability of the membranes was evaluated by measuring resistance of collagen membranes to collagenase digestion. Infrared spectra analyses of collagen films were performed in order to explore possible mechanisms of irradiation modification of collagen membranes. The results revealed that the degree of cross-linking and stability of collagen membranes after gamma-irradiation were improved. The MTT assay and SEM observation of the morphology of L929 mouse fibroblast cells which directly cultured on the collagen membranes were employed to evaluate the cytocompatibility of collagen membranes treated by gamma-ray radiation. In the range of 0 to approximately 25 KGy irradiation dose, no significant difference in cytocompatibility of collagen membranes irradiated by gamma-ray was observed. However, when the irradiation dose was over 25 KGy, the cytocompatibility of collagen membranes was influenced by gamma-radiation to some degree.
由牛皮胶原蛋白制备的膜在低温下分别接受15、25、35千戈瑞的γ射线辐射。使用的辐射剂量率约为22千戈瑞/小时。通过测量胶原膜对胶原酶消化的抗性来评估膜的稳定性。对胶原膜进行红外光谱分析，以探索胶原膜辐照改性的可能机制。结果表明，γ射线辐照后胶原膜的交联程度和稳定性得到提高。采用MTT法和扫描电子显微镜观察直接培养在胶原膜上的L929小鼠成纤维细胞的形态，以评估γ射线辐照处理的胶原膜的细胞相容性。在0至约25千戈瑞的辐照剂量范围内，未观察到γ射线辐照的胶原膜在细胞相容性方面有显著差异。然而，当辐照剂量超过25千戈瑞时，γ射线辐射在一定程度上影响了胶原膜的细胞相容性。
