Slichter Sherrill J, Bolgiano Doug, Jones Mary Kay, Christoffel Todd, Corson Jill, Rose Leslie, Foley Jim, Popovsky Mark, Baril Laurene L, Corda Tammy, Dincecco Dorothy M, Snyder Edward L
Puget Sound Blood Center, Seattle, Washington 98104-1256, USA.
Transfusion. 2006 Oct;46(10):1763-9. doi: 10.1111/j.1537-2995.2006.00970.x.
Methods of bacterial detection and pathogen inactivation of platelets (PLTs) may allow extended storage of PLTs as long as PLT quality is maintained.
Twenty normal volunteers had their PLTs collected with an apheresis machine (Haemonetics Corp.). A variety of in vitro PLT function and metabolic assays were performed both on Day 0 and after 8 days of storage. On Day 8, a small blood sample was drawn from each donor to obtain fresh PLTs. The fresh and stored autologous PLTs were labeled with either (51)Cr or (111)In, and the radiolabeled PLTs were transfused. Posttransfusion serial blood samples were drawn to determine the relative posttransfusion recoveries and survivals of the fresh versus the stored PLTs.
Although the in vitro assays showed some differences between the two trial sites, the results were generally within the ranges expected for fresh and stored PLTs. Overall, PLT recoveries averaged 66 +/- 16 percent versus 53 +/- 20 percent and survivals averaged 8.5 +/- 1.6 days versus 5.6 +/- 1.6 days, respectively, for fresh compared to 8-day-stored PLTs. There were no significant differences in the in vivo PLT data between the trial sites or based on the radiolabel used for the measurements.
After 8 days of storage, the in vivo posttransfusion recovery and survival of autologous Haemonetics apheresis PLTs meet the proposed standards for poststorage PLT quality.
只要血小板（PLT）质量得以维持，细菌检测和血小板病原体灭活方法可能允许延长血小板的储存时间。
20名正常志愿者通过血细胞分离机（Haemonetics公司）采集血小板。在第0天和储存8天后均进行了多种体外血小板功能和代谢检测。在第8天，从每位供者抽取少量血样以获取新鲜血小板。将新鲜和储存的自体血小板用（51）Cr或（111）In标记，然后输注放射性标记的血小板。输注后抽取系列血样以确定新鲜血小板与储存血小板输注后的相对回收率和存活率。
尽管体外检测显示两个试验地点之间存在一些差异，但结果总体上在新鲜和储存血小板预期的范围内。总体而言，与储存8天的血小板相比，新鲜血小板的回收率平均为66±16%，而储存血小板为53±20%；存活率平均为8.5±1.6天，而储存血小板为5.6±1.6天。试验地点之间或基于测量所用放射性标记的体内血小板数据均无显著差异。
储存8天后，自体Haemonetics血细胞分离血小板输注后的体内回收率和存活率符合储存后血小板质量的提议标准。
