Research Institute, Bloodworks Northwest, Seattle, Washington.
University of Washington School of Medicine, Seattle, Washington.
Transfusion. 2019 Jun;59(6):2084-2092. doi: 10.1111/trf.15302. Epub 2019 Apr 12.
Ordinarily, whole blood (WB) is separated into components before storage. We assessed the posttransfusion viability and function of platelets (PLTs) if they were stored within WB at 4°C.
Whole blood was obtained from 30 normal subjects and stored at 4°C without agitation for 12 days and for 10, 15, or 22 days with agitation. After WB storage, a PLT concentrate was prepared, and a fresh PLT sample was obtained from each donor. The stored PLTs were labeled with In and the fresh with Cr, and both were simultaneously transfused into their donor. Blood samples were obtained after transfusion to determine PLT recoveries and survivals. PLT samples from WB before and after storage were also assayed for PLT function and biochemistry.
After storage for 12 days without WB rotation, poststorage PLT counts averaged only 49 ± 12% of baseline values. After storage for 10, 15, or 22 days with end-over-end WB rotation, PLT counts averaged 76 ± 14% of baseline values. Fifteen-day poststorage radiolabeled PLT recoveries averaged 27 ± 11% (49 ± 16% of fresh), and survivals averaged 1.2 ± 0.4 days (16 ± 6% of fresh). in vitro assays demonstrated marked PLT activation after any storage time, and although PLT function decreased over time, stored PLTs were still considered acceptable.
These data suggest that, during rotated WB storage at 4°C for up to 15 days, PLT yields, poststorage PLT recoveries and survivals, and PLT function should be sufficient to support the short-term hemostatic needs of traumatized patients.
通常情况下，全血（WB）在储存前会分离成成分。我们评估了在 4°C 下储存在 WB 内的血小板（PLTs）的输注后活力和功能。
从 30 名正常受试者中获得全血，并在 4°C 下不搅拌储存 12 天，在搅拌下储存 10、15 或 22 天。在 WB 储存后，制备血小板浓缩物，并从每个供体获得新鲜血小板样本。储存的血小板用 In 标记，新鲜血小板用 Cr 标记，并同时将两者输注到供体中。输注后采集血液样本以确定血小板回收率和存活率。还检测了储存前后 WB 中血小板样本的血小板功能和生化特性。
在不旋转 WB 的情况下储存 12 天后，储存后的血小板计数平均仅为基线值的 49±12%。在旋转 WB 储存 10、15 或 22 天后，血小板计数平均为基线值的 76±14%。15 天后储存的放射性标记血小板回收率平均为 27±11%（新鲜血小板的 49±16%），存活率平均为 1.2±0.4 天（新鲜血小板的 16±6%）。体外检测表明，在任何储存时间后，血小板均发生明显激活，尽管血小板功能随时间下降，但储存的血小板仍被认为是可接受的。
这些数据表明，在 4°C 下旋转储存 WB 长达 15 天期间，血小板产量、储存后血小板回收率和存活率以及血小板功能应该足以满足创伤患者的短期止血需求。
